Abstract
We compared the results of routine blood tests for 102 blood donors' samples and 100 patients' samples collected in spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes to evaluate the impact of changes in formulation of the anticoagulant (K2EDTA vs. K3EDTA), its application (liquid vs. spray-dried), and tube material (glass vs. plastic). Methods for ABO/D testing, antibody screening, and antibody identification included direct hemagglutination/microplate (Olympus® PK 7200) and gel column methods (Ortho ID-Micro Typing System™/Gel Test™). Additional studies on blood donors' samples included time delayed antigen testing and antibody identification and half-draw/half-evacuated collections. Also, we compared the results of routine ABO/D testing and antibody screening for 50 patients' samples collected in spray-dried K2EDTA and spray-dried K 3EDTA and for an additional 50 patients' samples collected in spray-dried K2EDTA tubes from two different manufacturers. All patients' samples were tested in parallel by solid phase/microplate method (Immucor® ABS 2000) and the standard manual tube method. All test results for routine blood bank tests on donors' and patients' samples were concordant, demonstrating the equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes for routine donor center or transfusion service testing.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 117-121 |
| Number of pages | 5 |
| Journal | Immunohematology |
| Volume | 19 |
| Issue number | 4 |
| State | Published - Dec 12 2003 |
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Keywords
- Antibody screening
- Blood collection tubes
- Blood donors
- Blood typing
- EDTA
Cite this
Equivalence of spray-dried K2EDTA, spray-dried K 3EDTA, and liquid K3EDTA anticoagulated blood samples for routine blood center or transfusion service testing. / Leathem, Stacie; Zantek, Nicole Dodge; Kemper, Marti; Korte, Laura; Langeberg, Al; Sandler, S. G.
In: Immunohematology, Vol. 19, No. 4, 12.12.2003, p. 117-121.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Equivalence of spray-dried K2EDTA, spray-dried K 3EDTA, and liquid K3EDTA anticoagulated blood samples for routine blood center or transfusion service testing
AU - Leathem, Stacie
AU - Zantek, Nicole Dodge
AU - Kemper, Marti
AU - Korte, Laura
AU - Langeberg, Al
AU - Sandler, S. G.
PY - 2003/12/12
Y1 - 2003/12/12
N2 - We compared the results of routine blood tests for 102 blood donors' samples and 100 patients' samples collected in spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes to evaluate the impact of changes in formulation of the anticoagulant (K2EDTA vs. K3EDTA), its application (liquid vs. spray-dried), and tube material (glass vs. plastic). Methods for ABO/D testing, antibody screening, and antibody identification included direct hemagglutination/microplate (Olympus® PK 7200) and gel column methods (Ortho ID-Micro Typing System™/Gel Test™). Additional studies on blood donors' samples included time delayed antigen testing and antibody identification and half-draw/half-evacuated collections. Also, we compared the results of routine ABO/D testing and antibody screening for 50 patients' samples collected in spray-dried K2EDTA and spray-dried K 3EDTA and for an additional 50 patients' samples collected in spray-dried K2EDTA tubes from two different manufacturers. All patients' samples were tested in parallel by solid phase/microplate method (Immucor® ABS 2000) and the standard manual tube method. All test results for routine blood bank tests on donors' and patients' samples were concordant, demonstrating the equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes for routine donor center or transfusion service testing.
AB - We compared the results of routine blood tests for 102 blood donors' samples and 100 patients' samples collected in spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes to evaluate the impact of changes in formulation of the anticoagulant (K2EDTA vs. K3EDTA), its application (liquid vs. spray-dried), and tube material (glass vs. plastic). Methods for ABO/D testing, antibody screening, and antibody identification included direct hemagglutination/microplate (Olympus® PK 7200) and gel column methods (Ortho ID-Micro Typing System™/Gel Test™). Additional studies on blood donors' samples included time delayed antigen testing and antibody identification and half-draw/half-evacuated collections. Also, we compared the results of routine ABO/D testing and antibody screening for 50 patients' samples collected in spray-dried K2EDTA and spray-dried K 3EDTA and for an additional 50 patients' samples collected in spray-dried K2EDTA tubes from two different manufacturers. All patients' samples were tested in parallel by solid phase/microplate method (Immucor® ABS 2000) and the standard manual tube method. All test results for routine blood bank tests on donors' and patients' samples were concordant, demonstrating the equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes for routine donor center or transfusion service testing.
KW - Antibody screening
KW - Blood collection tubes
KW - Blood donors
KW - Blood typing
KW - EDTA
UR - http://www.scopus.com/inward/record.url?scp=0344981413&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0344981413&partnerID=8YFLogxK
M3 - Article
C2 - 15373676
AN - SCOPUS:0344981413
VL - 19
SP - 117
EP - 121
JO - Immunohematology / American Red Cross
JF - Immunohematology / American Red Cross
SN - 0894-203X
IS - 4
ER -