Epstein-Barr virus latent membrane protein 1 mediates phosphorylation and nuclear translocation of annexin A2 by activating PKC pathway

Guangrong Yan, Wei Luo, Zhongxin Lu, Xiangjian Luo, Lili Li, Sufang Liu, Yiping Liu, Min Tang, Zigang Dong, Ya Cao

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

We have previously combined phosphorylation enrichment with proteomics technology to elucidate the novel phosphoproteins in the signaling pathways triggered by Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) and shown that LMP1 can increase the phosphorylation level of annexin A2. Here, we further showed that LMP1 increased the serine, but not tyrosine, phosphorylation of annexin A2 by activating a novel signaling pathway, the protein kinase C (PKC) signaling pathway. However, LMP1 did not affect the level of annexin A2 expression. In addition, we found that LMP1 induced the nuclear entry of annexin A2 in an energy- and temperature-dependent manner, suggesting that the nuclear entry of annexin A2 is an active process. Treatment of LMP1-expressing cells with the PKC inhibitor myr-ψPKC resulted in annexin A2 being present almost exclusively at cell surface, instead of within the nucleus, suggesting that the nuclear entry of annexin A2 was associated with serine phosphorylation mediated by PKC.

Original languageEnglish (US)
Pages (from-to)341-348
Number of pages8
JournalCellular Signalling
Volume19
Issue number2
DOIs
StatePublished - Feb 1 2007

Keywords

  • Annexin A2
  • Latent membrane protein 1
  • Nuclear translocation
  • Phosphorylation
  • Protein kinase C

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