Epitope mapping of lymphocyte phosphatase-associated phosphoprotein

A. V. Filatov; T. D. Meshkova; D. V. Mazurov

doi:10.1134/S0006297914120153

Epitope mapping of lymphocyte phosphatase-associated phosphoprotein

A. V. Filatov, T. D. Meshkova, D. V. Mazurov

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Lymphocyte phosphatase-associated phosphoprotein (LPAP) is a transmembrane protein with unknown function. The available data on its close association with phosphatase CD45 and its phosphorylation depending on cell activation suggest that LPAP can play a significant role in the antigenic stimulation of lymphocytes. We have localized three antigenic epitopes of the LPAP molecule that can be detected using monoclonal antibodies prepared earlier. Experiments on reactions of antibodies with point mutants and shortened forms of the LPAP protein revealed regions of the amino acid sequence that correspond to the epitopes recognized by the antibodies.

Original language	English (US)
Pages (from-to)	1397-1404
Number of pages	8
Journal	Biochemistry (Moscow)
Volume	79
Issue number	12
DOIs	https://doi.org/10.1134/S0006297914120153
State	Published - Dec 2014
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Russian Foundation for Basic Research (project 12 04 00402) and the Russian Scientific Foundation (project 14 15 00702).

Publisher Copyright:
© 2014 Pleiades Publishing, Ltd.

Keywords

human lymphocytes
LPAP
monoclonal antibodies
site directed mutagenesis

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10.1134/S0006297914120153

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title = "Epitope mapping of lymphocyte phosphatase-associated phosphoprotein",

abstract = "Lymphocyte phosphatase-associated phosphoprotein (LPAP) is a transmembrane protein with unknown function. The available data on its close association with phosphatase CD45 and its phosphorylation depending on cell activation suggest that LPAP can play a significant role in the antigenic stimulation of lymphocytes. We have localized three antigenic epitopes of the LPAP molecule that can be detected using monoclonal antibodies prepared earlier. Experiments on reactions of antibodies with point mutants and shortened forms of the LPAP protein revealed regions of the amino acid sequence that correspond to the epitopes recognized by the antibodies.",

keywords = "human lymphocytes, LPAP, monoclonal antibodies, site directed mutagenesis",

author = "Filatov, {A. V.} and Meshkova, {T. D.} and Mazurov, {D. V.}",

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language = "English (US)",

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T1 - Epitope mapping of lymphocyte phosphatase-associated phosphoprotein

AU - Filatov, A. V.

AU - Meshkova, T. D.

AU - Mazurov, D. V.

N1 - Funding Information: This work was supported by the Russian Foundation for Basic Research (project 12 04 00402) and the Russian Scientific Foundation (project 14 15 00702). Publisher Copyright: © 2014 Pleiades Publishing, Ltd.

PY - 2014/12

Y1 - 2014/12

N2 - Lymphocyte phosphatase-associated phosphoprotein (LPAP) is a transmembrane protein with unknown function. The available data on its close association with phosphatase CD45 and its phosphorylation depending on cell activation suggest that LPAP can play a significant role in the antigenic stimulation of lymphocytes. We have localized three antigenic epitopes of the LPAP molecule that can be detected using monoclonal antibodies prepared earlier. Experiments on reactions of antibodies with point mutants and shortened forms of the LPAP protein revealed regions of the amino acid sequence that correspond to the epitopes recognized by the antibodies.

AB - Lymphocyte phosphatase-associated phosphoprotein (LPAP) is a transmembrane protein with unknown function. The available data on its close association with phosphatase CD45 and its phosphorylation depending on cell activation suggest that LPAP can play a significant role in the antigenic stimulation of lymphocytes. We have localized three antigenic epitopes of the LPAP molecule that can be detected using monoclonal antibodies prepared earlier. Experiments on reactions of antibodies with point mutants and shortened forms of the LPAP protein revealed regions of the amino acid sequence that correspond to the epitopes recognized by the antibodies.

KW - human lymphocytes

KW - LPAP

KW - monoclonal antibodies

KW - site directed mutagenesis

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JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

IS - 12

ER -

Epitope mapping of lymphocyte phosphatase-associated phosphoprotein

Abstract

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