TY - JOUR
T1 - Epithelial and neural localization and heparin binding of the cell-substratum adhesion molecule, epinectin
AU - Enenstein, J.
AU - Furcht, L. T.
PY - 1988/7
Y1 - 1988/7
N2 - Epinectin, a cell-substratum adhesion promoting molecule, was first isolated from the extracellular matrix of A431 human squamous carcinoma cells. In order to determine the biologic significance of epinectin, we determined the distribution of epinectin in various rat epithelial tissues by indirect immunofluorescence microscopy. Polyclonal antibodies to epinectin stained basal cells and basilar regions of skin, urinary bladder, and vagina. There was predominantly cytoplasmic staining along with amorphous extracellular staining. Strong staining was also noted in sebaceous glands and hair follicles. The immunoreactivity for epinectin in the skin was distinct from that for fibronectin, laminin, and type IV collagen. Antibodies to epinectin also stained subpopulations of neurons in the cerebrum and cerebellum. Epinectin antibodies strongly stained the cytoplasm of some pineal cells and cells of the pars intermedia of the pituitary. The distribution of epinectin suggests a role not only in epithelial cell-substratum adhesion, but in neuronal cell function. Heparan sulfate is known to be involved in the binding of several adhesion promoting molecules to cell surfaces. In order to assess the mechanism of adhesion of epinectin to cells, we measured the binding of 3H-heparin to epinectin. Binding of 3H-heparin was concentration dependent and inhibitable with cold heparin.
AB - Epinectin, a cell-substratum adhesion promoting molecule, was first isolated from the extracellular matrix of A431 human squamous carcinoma cells. In order to determine the biologic significance of epinectin, we determined the distribution of epinectin in various rat epithelial tissues by indirect immunofluorescence microscopy. Polyclonal antibodies to epinectin stained basal cells and basilar regions of skin, urinary bladder, and vagina. There was predominantly cytoplasmic staining along with amorphous extracellular staining. Strong staining was also noted in sebaceous glands and hair follicles. The immunoreactivity for epinectin in the skin was distinct from that for fibronectin, laminin, and type IV collagen. Antibodies to epinectin also stained subpopulations of neurons in the cerebrum and cerebellum. Epinectin antibodies strongly stained the cytoplasm of some pineal cells and cells of the pars intermedia of the pituitary. The distribution of epinectin suggests a role not only in epithelial cell-substratum adhesion, but in neuronal cell function. Heparan sulfate is known to be involved in the binding of several adhesion promoting molecules to cell surfaces. In order to assess the mechanism of adhesion of epinectin to cells, we measured the binding of 3H-heparin to epinectin. Binding of 3H-heparin was concentration dependent and inhibitable with cold heparin.
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U2 - 10.1111/1523-1747.ep12463285
DO - 10.1111/1523-1747.ep12463285
M3 - Article
C2 - 3290344
AN - SCOPUS:45449122128
SN - 0022-202X
VL - 91
SP - 34
EP - 38
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 1
ER -