Epigenetic alterations in metastatic cutaneous carcinoma

Owen A. Darr; Justin A. Colacino; Alice L. Tang; Jonathan B. McHugh; Emily L. Bellile; Carol R. Bradford; Mark P. Prince; Douglas B. Chepeha; Laura S. Rozek; Jeffrey S. Moyer

doi:10.1002/hed.23701

Epigenetic alterations in metastatic cutaneous carcinoma

Owen A. Darr
, Justin A. Colacino
, Alice L. Tang
, Jonathan B. McHugh
, Emily L. Bellile
, Carol R. Bradford
, Mark P. Prince
, Douglas B. Chepeha
, Laura S. Rozek
, Jeffrey S. Moyer

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Background Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) are the 2 most common cutaneous carcinomas. Molecular profiles predicting metastasis of these cancers have not been identified. Methods Epigenetic profiles of 37 primary cases of cutaneous SCC and BCC were quantified via the Illumina Goldengate Cancer Panel. Differential protein expression by metastatic potential was analyzed in 110 total cases by immunohistochemical (IHC) staining. Results Unsupervised hierarchical clustering analysis revealed that metastatic BCCs had a methylation profile resembling cutaneous SCCs. Metastatic cutaneous SCCs were found to be hypermethylated at FRZB (median methylation: 46.7% vs 4.7%; p = 4 × 10^-5). Metastatic BCCs were found to be hypomethylated at MYCL2 (median methylation: 3.8% vs 83.4%; p = 1.9 × 10^-6). Immunohistochemical staining revealed few differences between metastatic and nonmetastatic cancers. Conclusion Metastatic primary BCCs and cutaneous SCCs had distinct epigenetic profiles when compared to their nonmetastatic counterparts. Epigenetic profiling may prove useful in future diagnosis and prevention of advanced nonmelanoma skin cancers.

Original language	English (US)
Pages (from-to)	994-1001
Number of pages	8
Journal	Head and Neck
Volume	37
Issue number	7
DOIs	https://doi.org/10.1002/hed.23701
State	Published - Jul 1 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2014 Wiley Periodicals, Inc.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

basal
cutaneous carcinoma
DNA methylation
epigenetic
metastasis
squamous

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title = "Epigenetic alterations in metastatic cutaneous carcinoma",

abstract = "Background Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) are the 2 most common cutaneous carcinomas. Molecular profiles predicting metastasis of these cancers have not been identified. Methods Epigenetic profiles of 37 primary cases of cutaneous SCC and BCC were quantified via the Illumina Goldengate Cancer Panel. Differential protein expression by metastatic potential was analyzed in 110 total cases by immunohistochemical (IHC) staining. Results Unsupervised hierarchical clustering analysis revealed that metastatic BCCs had a methylation profile resembling cutaneous SCCs. Metastatic cutaneous SCCs were found to be hypermethylated at FRZB (median methylation: 46.7\% vs 4.7\%; p = 4 × 10-5). Metastatic BCCs were found to be hypomethylated at MYCL2 (median methylation: 3.8\% vs 83.4\%; p = 1.9 × 10-6). Immunohistochemical staining revealed few differences between metastatic and nonmetastatic cancers. Conclusion Metastatic primary BCCs and cutaneous SCCs had distinct epigenetic profiles when compared to their nonmetastatic counterparts. Epigenetic profiling may prove useful in future diagnosis and prevention of advanced nonmelanoma skin cancers.",

keywords = "basal, cutaneous carcinoma, DNA methylation, epigenetic, metastasis, squamous",

author = "Darr, \{Owen A.\} and Colacino, \{Justin A.\} and Tang, \{Alice L.\} and McHugh, \{Jonathan B.\} and Bellile, \{Emily L.\} and Bradford, \{Carol R.\} and Prince, \{Mark P.\} and Chepeha, \{Douglas B.\} and Rozek, \{Laura S.\} and Moyer, \{Jeffrey S.\}",

note = "Publisher Copyright: {\textcopyright} 2014 Wiley Periodicals, Inc.",

year = "2015",

month = jul,

day = "1",

doi = "10.1002/hed.23701",

language = "English (US)",

volume = "37",

pages = "994--1001",

journal = "Head and Neck",

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publisher = "John Wiley \& Sons Inc.",

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TY - JOUR

T1 - Epigenetic alterations in metastatic cutaneous carcinoma

AU - Darr, Owen A.

AU - Colacino, Justin A.

AU - Tang, Alice L.

AU - McHugh, Jonathan B.

AU - Bellile, Emily L.

AU - Bradford, Carol R.

AU - Prince, Mark P.

AU - Chepeha, Douglas B.

AU - Rozek, Laura S.

AU - Moyer, Jeffrey S.

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Background Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) are the 2 most common cutaneous carcinomas. Molecular profiles predicting metastasis of these cancers have not been identified. Methods Epigenetic profiles of 37 primary cases of cutaneous SCC and BCC were quantified via the Illumina Goldengate Cancer Panel. Differential protein expression by metastatic potential was analyzed in 110 total cases by immunohistochemical (IHC) staining. Results Unsupervised hierarchical clustering analysis revealed that metastatic BCCs had a methylation profile resembling cutaneous SCCs. Metastatic cutaneous SCCs were found to be hypermethylated at FRZB (median methylation: 46.7% vs 4.7%; p = 4 × 10-5). Metastatic BCCs were found to be hypomethylated at MYCL2 (median methylation: 3.8% vs 83.4%; p = 1.9 × 10-6). Immunohistochemical staining revealed few differences between metastatic and nonmetastatic cancers. Conclusion Metastatic primary BCCs and cutaneous SCCs had distinct epigenetic profiles when compared to their nonmetastatic counterparts. Epigenetic profiling may prove useful in future diagnosis and prevention of advanced nonmelanoma skin cancers.

AB - Background Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) are the 2 most common cutaneous carcinomas. Molecular profiles predicting metastasis of these cancers have not been identified. Methods Epigenetic profiles of 37 primary cases of cutaneous SCC and BCC were quantified via the Illumina Goldengate Cancer Panel. Differential protein expression by metastatic potential was analyzed in 110 total cases by immunohistochemical (IHC) staining. Results Unsupervised hierarchical clustering analysis revealed that metastatic BCCs had a methylation profile resembling cutaneous SCCs. Metastatic cutaneous SCCs were found to be hypermethylated at FRZB (median methylation: 46.7% vs 4.7%; p = 4 × 10-5). Metastatic BCCs were found to be hypomethylated at MYCL2 (median methylation: 3.8% vs 83.4%; p = 1.9 × 10-6). Immunohistochemical staining revealed few differences between metastatic and nonmetastatic cancers. Conclusion Metastatic primary BCCs and cutaneous SCCs had distinct epigenetic profiles when compared to their nonmetastatic counterparts. Epigenetic profiling may prove useful in future diagnosis and prevention of advanced nonmelanoma skin cancers.

KW - basal

KW - cutaneous carcinoma

KW - DNA methylation

KW - epigenetic

KW - metastasis

KW - squamous

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U2 - 10.1002/hed.23701

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AN - SCOPUS:84931573599

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JO - Head and Neck

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ER -

Epigenetic alterations in metastatic cutaneous carcinoma

Abstract

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