Enzyme-targeted fluorescent small-molecule probes for bacterial imaging

Andrew P. Marshall, Joshua D. Shirley, Erin E. Carlson

Research output: Contribution to journalReview articlepeer-review

Abstract

Molecular imaging methods to visualize myriad biochemical processes in bacteria have traditionally been dependent upon molecular biology techniques to incorporate fluorescent biomolecules (e.g., fusion proteins). Such methods have been instrumental in our understanding of how bacteria function but are not without drawbacks, including potential perturbation to native protein expression and function. To overcome these limitations, the use of fluorescent small-molecule probes has gained much attention. Here, we highlight examples from the recent literature that showcase the utility of small-molecule probes for the fluorescence imaging of bacterial cells, including electrophilic, metabolic, and enzyme-activated probes. Although the use of these types of compounds for bacterial imaging is still relatively new, the selected examples demonstrate the exciting potential of these critical tools in the exploration of bacterial physiology.

Original languageEnglish (US)
Pages (from-to)155-165
Number of pages11
JournalCurrent opinion in chemical biology
Volume57
DOIs
StatePublished - Aug 2020

Bibliographical note

Funding Information:
This work was supported by the National Institutes of Health (R01 GM128439-01A1 to EEC.) and the Department of Chemistry of the University of Minnesota. JDS. was supported by the National Institutes of Health's National Center for Advancing Translational Sciences, grants TL1R002493 and UL1TR002494. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health's National Center for Advancing Translational Sciences.

Funding Information:
This work was supported by the National Institutes of Health (R01 GM128439-01A1 to EEC.) and the Department of Chemistry of the University of Minnesota . JDS. was supported by the National Institutes of Health's National Center for Advancing Translational Sciences , grants TL1R002493 and UL1TR002494 . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health's National Center for Advancing Translational Sciences.

Keywords

  • Activity-based probe
  • Bacteria
  • Chemical probe
  • Electrophile
  • Fluorescence imaging

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