Enzyme nanorings

Tsui Fen Chou, Christopher So, Brian R. White, Jonathan C.T. Carlson, Mehmet Sarikaya, Carston R Wagner

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We have demonstrated that nanostructures, and in particular nanorings incorporating a homodimeric enzyme, can be prepared by chemically induced self-assembly of dihydrofolate reductase (DHFR)histidine triad nucleotide binding 1 (Hint1) fusion proteins. The dimensions of the nanorings were found by static light scattering and atomicforce microscopy studies to be dependent on the length and composition of the peptide linking the fusion proteins, ranging in size from 10 to 70 nm in diameter and 64 to 740 kDa. The catalytic efficiency of the nanorings was found to be dependent on ring size, thus suggesting that the arrangement of supermolecular assemblies of enzymes may be used to control their catalytic parameters.

Original languageEnglish (US)
Pages (from-to)2519-2525
Number of pages7
JournalACS Nano
Volume2
Issue number12
DOIs
StatePublished - Dec 23 2008

Fingerprint

Nanorings
enzymes
Enzymes
fusion
proteins
histidine
nucleotides
Fusion reactions
assemblies
peptides
Proteins
self assembly
Tetrahydrofolate Dehydrogenase
light scattering
Nucleotides
microscopy
Histidine
Light scattering
Self assembly
Peptides

Keywords

  • Chemical dimerization
  • Dihydrofolate reductase (DHFR)
  • Histidine triad nucleotide binding proteins (Hints)
  • Human hint1
  • Nanoring
  • Phosphoramidase
  • Self-assembly

Cite this

Chou, T. F., So, C., White, B. R., Carlson, J. C. T., Sarikaya, M., & Wagner, C. R. (2008). Enzyme nanorings. ACS Nano, 2(12), 2519-2525. https://doi.org/10.1021/nn800577h

Enzyme nanorings. / Chou, Tsui Fen; So, Christopher; White, Brian R.; Carlson, Jonathan C.T.; Sarikaya, Mehmet; Wagner, Carston R.

In: ACS Nano, Vol. 2, No. 12, 23.12.2008, p. 2519-2525.

Research output: Contribution to journalArticle

Chou, TF, So, C, White, BR, Carlson, JCT, Sarikaya, M & Wagner, CR 2008, 'Enzyme nanorings', ACS Nano, vol. 2, no. 12, pp. 2519-2525. https://doi.org/10.1021/nn800577h
Chou TF, So C, White BR, Carlson JCT, Sarikaya M, Wagner CR. Enzyme nanorings. ACS Nano. 2008 Dec 23;2(12):2519-2525. https://doi.org/10.1021/nn800577h
Chou, Tsui Fen ; So, Christopher ; White, Brian R. ; Carlson, Jonathan C.T. ; Sarikaya, Mehmet ; Wagner, Carston R. / Enzyme nanorings. In: ACS Nano. 2008 ; Vol. 2, No. 12. pp. 2519-2525.
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