Abstract
An enzyme-linked immunosorbent assay (ELISA) to quantitate anti-lipid A antibodies in sera has been developed. The sensitivity of the ELISA was improved when the antigen (lipid A) was immobilized at pH 2.0, presumably by enhanced solid-phase adsorption of lipid A which is presumed to be aggregated at low pH. This was also verified by solid-phase immunoradiometry in which a four-fold improvement in the signal-to-noise ratio was observed when antigen coating was performed at pH 2.0 as compared to coating at pH 9.6. The enhanced sensitivity permitted the use of low concentrations of lipid A (10 μg/ml) for antigen coating of microtiter plates. The assay was able to clearly detect differences in IgG anti-lipid A levels between patients with chronic liver disease and normal controls.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 85-89 |
| Number of pages | 5 |
| Journal | Journal of Immunological Methods |
| Volume | 163 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 6 1993 |
Bibliographical note
Funding Information:Research on Enteric Diseases by the Wellcome Research Laboratory is supported by the Indian Council of Medical Research, New Delhi. This work was also supported by grants from the Wellcome Trust, London, UK, and the Rockefeller Foundation, New York, USA. SD is a recipient of a Senior Research Fellowship from The Council for Scientific and, Industrial Research, Government of India. The authors wish to thank Mr. Rishi Raj.
Keywords
- ELISA
- Lipid A
- Lipopolysaccharide