Enzymatically synthesised fructooligosaccharides from sugarcane syrup modulate the composition and short-chain fatty acid production of the human intestinal microbiota

Siti Hajar-Azhari, Muhamad Hafiz Abd Rahim, Shahrul Razid Sarbini, Belal J. Muhialdin, Lasekan Olusegun, Nazamid Saari

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Fructooligosaccharides can be produced by direct enzymatic conversion from sucrose-rich sugarcane syrup (SS) consisting of 58.93% sucrose yielding 21.28 g FOS/100 g sucrose. This study evaluated the prebiotic effect of unpurified/purified SS containing FOS for the modulation of the human intestinal microbial composition and short-chain fatty acid production. The unpurified and purified FOS substrates, which were a mixture of 1-kestose, nystose and 1F-fructosylnystose, were supplemented into human faecal culture using a pH-controlled batch fermentation system and significantly increased the Bifidobacterium counts after 5 h fermentation, while Bacteroides/Prevotella counts were highest throughout 24 h fermentation. Meanwhile, Lactobacillus/Enterococcus exhibited a slight increase after 5 h fermentation before reaching a plateau afterwards. The steady Bacteroides/Prevotella growth and increased Bifidobacterium population promoted an increase in the production of short-chain fatty acids acetate (58 ± 2.70 mM), propionate (9.19 ± 5.94 mM) and butyrate (7.15 ± 2.28 mM). These results provide evidence that representative gut microbiota could utilise the enzymatically synthesised FOS to generate short-chain fatty acids as metabolites in pH-controlled conditions, thus FOS from SS are a potential prebiotic ingredient for foods and health drinks.

Original languageEnglish (US)
Article number110677
JournalFood Research International
Volume149
DOIs
StatePublished - Nov 2021
Externally publishedYes

Bibliographical note

Funding Information:
We would like to extend our gratitude to Universiti Putra Malaysia (UPM) for the financial support awarded to Prof Dr Nazamid Saari under Dana Tautan (Vot No: 9200902).

Publisher Copyright:
© 2021

Keywords

  • Enzymatic synthesis
  • Fructooligosaccharides
  • Prebiotic
  • SCFA
  • Sugarcane

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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