Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening

Andrew B. Lane; Magdalena Strzelecka; Andreas Ettinger; Andrew W. Grenfell; Torsten Wittmann; Rebecca Heald

doi:10.1016/j.devcel.2015.06.003

Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening

Andrew B. Lane, Magdalena Strzelecka, Andreas Ettinger, Andrew W. Grenfell, Torsten Wittmann, Rebecca Heald

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

CRISPR-based technologies have emerged as powerful tools to alter genomes and mark chromosomal loci, but an inexpensive method for generating large numbers of RNA guides for whole genome screening and labeling is lacking. Using a method that permits library construction from any source of DNA, we generated guide libraries that label repetitive loci or a single chromosomal locus in Xenopus egg extracts and show that a complex library can target the E. coli genome at high frequency.

Original language	English (US)
Pages (from-to)	373-378
Number of pages	6
Journal	Developmental Cell
Volume	34
Issue number	3
DOIs	https://doi.org/10.1016/j.devcel.2015.06.003
State	Published - Aug 10 2015
Externally published	Yes

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© 2015 Elsevier Inc.

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AU - Wittmann, Torsten

AU - Heald, Rebecca

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AB - CRISPR-based technologies have emerged as powerful tools to alter genomes and mark chromosomal loci, but an inexpensive method for generating large numbers of RNA guides for whole genome screening and labeling is lacking. Using a method that permits library construction from any source of DNA, we generated guide libraries that label repetitive loci or a single chromosomal locus in Xenopus egg extracts and show that a complex library can target the E. coli genome at high frequency.

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Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening

Abstract

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