Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening

Andrew B. Lane, Magdalena Strzelecka, Andreas Ettinger, Andrew W. Grenfell, Torsten Wittmann, Rebecca Heald

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


CRISPR-based technologies have emerged as powerful tools to alter genomes and mark chromosomal loci, but an inexpensive method for generating large numbers of RNA guides for whole genome screening and labeling is lacking. Using a method that permits library construction from any source of DNA, we generated guide libraries that label repetitive loci or a single chromosomal locus in Xenopus egg extracts and show that a complex library can target the E. coli genome at high frequency.

Original languageEnglish (US)
Pages (from-to)373-378
Number of pages6
JournalDevelopmental Cell
Issue number3
StatePublished - Aug 10 2015

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