Enzymatic synthesis of L-lactic acid from carbon dioxide and ethanol with an inherent cofactor regeneration cycle

Xiaodong Tong, Bilal El-Zahab, Xueyan Zhao, Youyan Liu, Ping Wang

Research output: Contribution to journalLetter

40 Scopus citations

Abstract

Efficient conversion of carbon dioxide is of great interests to today's endeavors in controlling greenhouse gas emission. A multienzyme catalytic system that uses carbon dioxide and ethanol to produce L-lactate was demonstrated in this work, thereby providing a novel reaction route to convert bio-based ethanol to an important building block for synthesis biodegradable polymers. The synthetic route has a unique internal cofactor regeneration cycle, eliminating the need of additional chemical or energy for cofactor regeneration. Lactate was successfully synthesized with 41% of ethanol converted in a batch reaction, while a turnover number of 2.2 day-1 was reached for cofactor regeneration in a reaction with continuous feeding of ethanol. A kinetic model developed based on reaction kinetic parameters determined separately for each reaction step predicted well the reaction rates and yields of the multienzyme reaction system. Biotechnol. Bioeng. 2011;108: 465-469.

Original languageEnglish (US)
Pages (from-to)465-469
Number of pages5
JournalBiotechnology and bioengineering
Volume108
Issue number2
DOIs
StatePublished - Feb 1 2011

Keywords

  • Carbon dioxide
  • Cofactor regeneration
  • Enzymatic biocatalysis
  • L-lactic acid
  • Lactate dehydrogenase

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