Enzymatic labeling of proteins

Techniques and approaches

Mohammad Rashidian, Jonathan K. Dozier, Mark D Distefano

Research output: Contribution to journalReview article

143 Citations (Scopus)

Abstract

Site-specific modification of proteins is a major challenge in modern chemical biology due to the large number of reactive functional groups typically present in polypeptides. Because of its importance in biology and medicine, the development of methods for site-specific modification of proteins is an area of intense research. Selective protein modification procedures have been useful for oriented protein immobilization, for studies of naturally occurring post-translational modifications, for creating antibody-drug conjugates, for the introduction of fluorophores and other small molecules on to proteins, for examining protein structure, folding, dynamics, and protein-protein interactions, and for the preparation of protein-polymer conjugates. One of the most important approaches for protein labeling is to incorporate bioorthogonal functionalities into proteins at specific sites via enzymatic reactions. The incorporated tags then enable reactions that are chemoselective, whose functional groups not only are inert in biological media, but also do not occur natively in proteins or other macromolecules. This review article summarizes the enzymatic strategies, which enable site-specific functionalization of proteins with a variety of different functional groups. The enzymes covered in this review include formylglycine generating enzyme, sialyltransferases, phosphopantetheinyltransferases, O-GlcNAc post-translational modification, sortagging, transglutaminase, farnesyltransferase, biotin ligase, lipoic acid ligase, and N-myristoyltransferase.

Original languageEnglish (US)
Pages (from-to)1277-1294
Number of pages18
JournalBioconjugate Chemistry
Volume24
Issue number8
DOIs
StatePublished - Aug 21 2013

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Labeling
Proteins
Functional groups
Post Translational Protein Processing
Ligases
Enzymes
Sialyltransferases
Farnesyltranstransferase
Thioctic Acid
Transglutaminases
Protein Folding
Fluorophores
Polypeptides
Biotin
Immobilization
Macromolecules
Antibodies
Medicine
Polymers
Peptides

Cite this

Enzymatic labeling of proteins : Techniques and approaches. / Rashidian, Mohammad; Dozier, Jonathan K.; Distefano, Mark D.

In: Bioconjugate Chemistry, Vol. 24, No. 8, 21.08.2013, p. 1277-1294.

Research output: Contribution to journalReview article

Rashidian, Mohammad ; Dozier, Jonathan K. ; Distefano, Mark D. / Enzymatic labeling of proteins : Techniques and approaches. In: Bioconjugate Chemistry. 2013 ; Vol. 24, No. 8. pp. 1277-1294.
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