Removal of the chorion from fish eggs facilitates observation of piscine embryogenesis and allows efficient microinjection during gene transfer protocols. Several treatments were screened to evaluate their effectiveness in removing the chorion from newly fertilized eggs of goldfish Carassius auratus. walleye Stizostedion vitreum, and northern pike Esox lucius without destroying egg viability. Goldfish eggs dechorionated in 2.5 mg/mL trypsin showed excellent viability. No effective procedure for dechorionation of walleye eggs was found. Northern pike eggs could be dechorionated in 0.6 mg/mL protease type XXV, but mortalities above 50% were commonly observed.
|Original language||English (US)|
|Number of pages||5|
|Journal||Transactions of the American Fisheries Society|
|State||Published - Sep 1988|
Bibliographical noteFunding Information:
We gratefully acknowledge the cooperation of the Oklahoma Department of Wildlife Conservation, the Colorado Department of Natural Re-sources, the Kansas Fish and Game Commission, and the Minnesota Department of Natural Re- sources in the acquisitions of walleye gametes. We thank Ron Rademacher (Rademacher Ponds) and the Minnesota Department of Natural Resources for provision of northern pike gametes. Jay Ma-her, Michael Cashman, Dimuthu DeSilva, and Es- ter Hallerman provided technical assistance. This research was supported by grants from the Leg- islative Commission for Minnesota Resources of the state of Minnesota, the Minnesota Agricultural Experiment Station, and Minnesota Sea Grant. This is contribution 16,094 of the Minnesota Ag- ricultural Experiment Station scientific journal ar- ticle series and Minnesota Sea Grant journal re- print 217. The Minnesota Sea Grant College Program is supported by the Office of Sea Grant, Department of Commerce, under grant NA85AA-D-SG112.