Entropic trap-based tunable short-pass filter to recover long DNA for genomic applications

Pranav Agrawal, Zsófia Bognár, Kevin D. Dorfman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed an entropic-based device that filters short DNA to improve sample quality for downstream genetic applications like genome mapping and genome sequencing, providing a marked improvement over conventional methods such as gel electrophoresis. We use microchannel/nanoslit interfaces to sculpt the electric field, thereby trapping long DNA and removing the short DNA. Furthermore, a pressure-driven flow in the microchannel injects and removes DNA from the interface for continuous filtration. Our data demonstrates that this device could both concentrate rare genomic DNA samples and remove impurities in next-generation genomics applications.

Original languageEnglish (US)
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages1346-1347
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - Jan 1 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period10/22/1710/26/17

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Keywords

  • Electrophoresis
  • Entropic trap
  • Filtration
  • Lab-on-a-chip
  • Long DNA
  • Microchannel
  • Nanoslits

Cite this

Agrawal, P., Bognár, Z., & Dorfman, K. D. (2020). Entropic trap-based tunable short-pass filter to recover long DNA for genomic applications. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 1346-1347). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.