Enterococcal PrgU Provides Additional Regulation of Pheromone-Inducible Conjugative Plasmids

Lena Lassinantti, Martha I. Camacho, Rebecca J.B. Erickson, Julia L.E. Willett, Nicholas R. De Lay, Josy Ter Beek, Gary M. Dunny, Peter J. Christie, Ronnie P.A. Berntsson

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Efficient horizontal gene transfer of the conjugative plasmid pCF10 from Enterococcus faecalis depends on the expression of its type 4 secretion system (T4SS) genes, controlled by the PQ promoter. Transcription from the PQ promoter is tightly regulated, partially to limit cell toxicity caused by overproduction of PrgB, a T4SS adhesin. PrgU plays an important role in regulating this toxicity by decreasing PrgB levels. PrgU has an RNA-binding fold, prompting us to test whether PrgU exerts its regulatory control through binding of prgQ transcripts. We used a combination of in vivo methods to quantify PrgU effects on prgQ transcripts at both single-cell and population levels. PrgU function requires a specific RNA sequence within an intergenic region (IGR) about 400 bp downstream of PQ. PrgU interaction with the IGR reduces levels of downstream transcripts. Single-cell expression analysis showed that cells expressing prgU decreased transcript levels more rapidly than isogenic prgU-minus cells. PrgU bound RNA in vitro without sequence specificity, suggesting that PrgU requires a specific RNA structure or one or more host factors for selective binding in vivo. PrgU binding to its IGR target might recruit RNase(s) for targeted degradation of downstream transcripts or reduce elongation of nascent transcripts beyond the IGR.

Original languageEnglish (US)
Article numbere00264-21
JournalmSphere
Volume6
Issue number3
DOIs
StatePublished - May 2021

Bibliographical note

Funding Information:
This work was supported by grants from the Swedish Research Council (2016-03599), Knut and Alice Wallenberg Foundation, and Kempestiftelserna (SMK-1869) to R.P.-A.B. and by NIH grant R35GM118079 to G.M.D. and grants R01GM48476 and R35GM131892 to P.J.C.

Publisher Copyright:
© 2021 Lassinantti et al.

Keywords

  • Conjugation
  • Regulation
  • Type 4 secretion systems

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

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