Enterococcal Aggregation Substance and Binding Substance Are Not Major Contributors to Urinary Tract Colonization by Enterococcus faecalis in A Mouse Model of Ascending Unobstructed Urinary Tract Infection

James R. Johnson; Connie Clabots; Helmut Hirt; Christopher Waters; Gary Dunny

doi:10.1128/IAI.72.4.2445-2448.2004

Enterococcal Aggregation Substance and Binding Substance Are Not Major Contributors to Urinary Tract Colonization by Enterococcus faecalis in A Mouse Model of Ascending Unobstructed Urinary Tract Infection

James R. Johnson, Connie Clabots, Helmut Hirt, Christopher Waters, Gary Dunny

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Isogenic Enterococcus faecalis strains that differ in their expression of aggregation substance (AS) and its cognate receptor, enterococcal binding substance (EBS), were compared for urovirulence in mice. Strain OG1SSp/pCF500 (inducible AS⁺, constitutive EBS⁺) failed to outcompete isogenic derivative INY3000 (AS^- EBS^-) in the urine, bladders, or kidneys of mice harvested at 48 h postinoculation. Neither mouse nor human urine induced AS expression by OG1SSp/pCF500. Recombinant strain OG1SSp/pINY1801 (constitutive AS⁺, EBS⁺) plasmid segregation that was as extensive in vivo as in vitro. These data suggest that AS and EBS do not contribute to upper or lower urinary tract colonization by E. faecalis and that growth in urine does not induce AS expression by strains carrying plasmids in the pCF10 family.

Original language	English (US)
Pages (from-to)	2445-2448
Number of pages	4
Journal	Infection and immunity
Volume	72
Issue number	4
DOIs	https://doi.org/10.1128/IAI.72.4.2445-2448.2004
State	Published - Apr 2004

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Enterococcal Aggregation Substance and Binding Substance Are Not Major Contributors to Urinary Tract Colonization by Enterococcus faecalis in A Mouse Model of Ascending Unobstructed Urinary Tract Infection. / Johnson, James R.; Clabots, Connie; Hirt, Helmut et al.

In: Infection and immunity, Vol. 72, No. 4, 04.2004, p. 2445-2448.

Research output: Contribution to journal › Article › peer-review

Johnson, JR, Clabots, C, Hirt, H, Waters, C & Dunny, G 2004, 'Enterococcal Aggregation Substance and Binding Substance Are Not Major Contributors to Urinary Tract Colonization by Enterococcus faecalis in A Mouse Model of Ascending Unobstructed Urinary Tract Infection', Infection and immunity, vol. 72, no. 4, pp. 2445-2448. https://doi.org/10.1128/IAI.72.4.2445-2448.2004

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abstract = "Isogenic Enterococcus faecalis strains that differ in their expression of aggregation substance (AS) and its cognate receptor, enterococcal binding substance (EBS), were compared for urovirulence in mice. Strain OG1SSp/pCF500 (inducible AS+, constitutive EBS+) failed to outcompete isogenic derivative INY3000 (AS- EBS-) in the urine, bladders, or kidneys of mice harvested at 48 h postinoculation. Neither mouse nor human urine induced AS expression by OG1SSp/pCF500. Recombinant strain OG1SSp/pINY1801 (constitutive AS+, EBS+) plasmid segregation that was as extensive in vivo as in vitro. These data suggest that AS and EBS do not contribute to upper or lower urinary tract colonization by E. faecalis and that growth in urine does not induce AS expression by strains carrying plasmids in the pCF10 family.",

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AB - Isogenic Enterococcus faecalis strains that differ in their expression of aggregation substance (AS) and its cognate receptor, enterococcal binding substance (EBS), were compared for urovirulence in mice. Strain OG1SSp/pCF500 (inducible AS+, constitutive EBS+) failed to outcompete isogenic derivative INY3000 (AS- EBS-) in the urine, bladders, or kidneys of mice harvested at 48 h postinoculation. Neither mouse nor human urine induced AS expression by OG1SSp/pCF500. Recombinant strain OG1SSp/pINY1801 (constitutive AS+, EBS+) plasmid segregation that was as extensive in vivo as in vitro. These data suggest that AS and EBS do not contribute to upper or lower urinary tract colonization by E. faecalis and that growth in urine does not induce AS expression by strains carrying plasmids in the pCF10 family.

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Enterococcal Aggregation Substance and Binding Substance Are Not Major Contributors to Urinary Tract Colonization by Enterococcus faecalis in A Mouse Model of Ascending Unobstructed Urinary Tract Infection

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