Ensembles of engineered cardiac tissues for physiological and pharmacological study: Heart on a chip

Anna Grosberg, Patrick W. Alford, Megan L. McCain, Kevin Kit Parker

Research output: Contribution to journalArticlepeer-review

350 Scopus citations

Abstract

Traditionally, muscle physiology experiments require multiple tissue samples to obtain morphometric, electrophysiological, and contractility data. Furthermore, these experiments are commonly completed one at a time on cover slips of single cells, isotropic monolayers, or in isolated muscle strips. In all of these cases, variability of the samples hinders quantitative comparisons among experimental groups. Here, we report the design of a "heart on a chip" that exploits muscular thin film technology - biohybrid constructs of an engineered, anisotropic ventricular myocardium on an elastomeric thin film - to measure contractility, combined with a quantification of action potential propagation, and cytoskeletal architecture in multiple tissues in the same experiment. We report techniques for real-time data collection and analysis during pharmacological intervention. The chip is an efficient means of measuring structure-function relationships in constructs that replicate the hierarchical tissue architectures of laminar cardiac muscle.

Original languageEnglish (US)
Pages (from-to)4165-4173
Number of pages9
JournalLab on a chip
Volume11
Issue number24
DOIs
StatePublished - Dec 21 2011

Fingerprint Dive into the research topics of 'Ensembles of engineered cardiac tissues for physiological and pharmacological study: Heart on a chip'. Together they form a unique fingerprint.

Cite this