Minichromosome maintenance protein 10 (Mcm10) is required for DNA replication in all eukaryotes. Although the exact contribution of Mcm10 to genome replication remains heavily debated, early reports suggested that it promotes DNA unwinding and origin firing. These ideas have been solidified by recent studies that propose a role for Mcm10 in helicase activation. Whereas the molecular underpinnings of this activation step have yet to be revealed, structural data on Mcm10 provide further insight into a possible mechanism of action. The essential role in DNA replication initiation is not mutually exclusive with additional functions that Mcm10 may have as part of the elongation machinery. Here, we review the recent findings regarding the role of Mcm10 in DNA replication and discuss existing controversies.
|Original language||English (US)|
|Number of pages||11|
|Journal||Trends in Biochemical Sciences|
|State||Published - Apr 2013|
Bibliographical noteFunding Information:
The authors wish to acknowledge funding from the National Institutes of Health (NIH) (GM074917) and the Leukemia and Lymphoma Society to A-K.B. and thank Dr Eric Hendrickson for proofreading of the manuscript.
- DNA replication
- Double-strand breaks
- Genome integrity
- Replication forks
- Zn-finger domains