Enhancing the sensitivity of multidimensional NMR experiments by using triply-compensated π pulses

Youlin Xia, Paolo Rossi, Manu V. Subrahmanian, Chengdong Huang, Tamjeed Saleh, Cristina Olivieri, Charalampos G. Kalodimos, Gianluigi Veglia

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


In multidimensional solution NMR experiments, π pulses are used extensively for inversion and refocusing operations on 1H, 13C and 15N nuclei. Pulse miscalibration, off-resonance effects, and J-coupling evolution during π pulse execution result in severe signal losses that are exacerbated at high magnetic fields. Here, we report the implementation of a triply-compensated π pulse (G5) optimized for both inversion and refocusing in widely used 2- and 3-dimensional experiments. By replacing most of the hard π pulses, adiabatic or composite pulses on the 1H, 13C and 15N channels with G5 pulses, we obtained signal enhancements ranging from 80 to 240%. We anticipate that triply-compensated pulses will be crucial for improving the performance of multidimensional and multinuclear pulse sequences at ultra-high fields.

Original languageEnglish (US)
Pages (from-to)237-243
Number of pages7
JournalJournal of biomolecular NMR
Issue number4
StatePublished - Dec 1 2017

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media B.V., part of Springer Nature.


  • Genetic algorithm
  • Off-resonance effects
  • Sensitivity enhancement
  • Triply compensated pulses (G5)
  • Universal inversion and refocusing pulses


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