TY - JOUR
T1 - Enhancing the sensitivity of multidimensional NMR experiments by using triply-compensated π pulses
AU - Xia, Youlin
AU - Rossi, Paolo
AU - Subrahmanian, Manu V.
AU - Huang, Chengdong
AU - Saleh, Tamjeed
AU - Olivieri, Cristina
AU - Kalodimos, Charalampos G.
AU - Veglia, Gianluigi
N1 - Publisher Copyright:
© 2017, Springer Science+Business Media B.V., part of Springer Nature.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - In multidimensional solution NMR experiments, π pulses are used extensively for inversion and refocusing operations on 1H, 13C and 15N nuclei. Pulse miscalibration, off-resonance effects, and J-coupling evolution during π pulse execution result in severe signal losses that are exacerbated at high magnetic fields. Here, we report the implementation of a triply-compensated π pulse (G5) optimized for both inversion and refocusing in widely used 2- and 3-dimensional experiments. By replacing most of the hard π pulses, adiabatic or composite pulses on the 1H, 13C and 15N channels with G5 pulses, we obtained signal enhancements ranging from 80 to 240%. We anticipate that triply-compensated pulses will be crucial for improving the performance of multidimensional and multinuclear pulse sequences at ultra-high fields.
AB - In multidimensional solution NMR experiments, π pulses are used extensively for inversion and refocusing operations on 1H, 13C and 15N nuclei. Pulse miscalibration, off-resonance effects, and J-coupling evolution during π pulse execution result in severe signal losses that are exacerbated at high magnetic fields. Here, we report the implementation of a triply-compensated π pulse (G5) optimized for both inversion and refocusing in widely used 2- and 3-dimensional experiments. By replacing most of the hard π pulses, adiabatic or composite pulses on the 1H, 13C and 15N channels with G5 pulses, we obtained signal enhancements ranging from 80 to 240%. We anticipate that triply-compensated pulses will be crucial for improving the performance of multidimensional and multinuclear pulse sequences at ultra-high fields.
KW - Genetic algorithm
KW - Off-resonance effects
KW - Sensitivity enhancement
KW - Triply compensated pulses (G5)
KW - Universal inversion and refocusing pulses
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U2 - 10.1007/s10858-017-0153-2
DO - 10.1007/s10858-017-0153-2
M3 - Article
C2 - 29164453
AN - SCOPUS:85034630462
SN - 0925-2738
VL - 69
SP - 237
EP - 243
JO - Journal of biomolecular NMR
JF - Journal of biomolecular NMR
IS - 4
ER -