It was previously believed that the neuronal type of nitric oxide (NO) synthase was constitutive in nature, and that changes in the concentration of intracellular Ca2+ represent the sole input that regulates its activity. Recent reports, however, suggested that this enzyme could also be induced under certain conditions. We report here that prolonged stimulation of M1 muscarinic acetylcholine receptors results in potentiation of maximal receptor-mediated activation of neuronal NO synthase in Chinese hamster ovary cells. This effect was dependent on the concentration of agonist during the treatment and was abolished by a muscarinic receptor antagonist. These findings are important for understanding the sequelae of prolonged administration of muscarinic agonists in vivo.
Bibliographical noteFunding Information:
The authors thank Sheng Zu Zhu for the preparation of the transfected cell line used in this study. This work was supported by NIH grant NS25743.
- Intact cells
- Muscarinic acetylcholine receptors
- Nitric oxide (NO)
- Nitric oxide (NO) synthase