Enhancement of lutein biosynthesis in chlorella vulgaris-1068 under low temperature and luminosity stress conditions

Wanqing Wang, Mingyang Chen, Jie Liu, Wei Hua, Shuang Wu, Mengjie Diao, Yanling Cheng

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Lutein, a high-value primary carotenoid with a broad range of applications in food, feed, nutraceutical, and pharmaceutical industries, has been widely studied in recent years. The green microalgae Chlorella vulgaris-1068(C.v-1068) screened by our lab showed both a high content in this carotenoid and a high growth rate and has a good profile for lutein production. This work studies the variety of environmental stimulants which enhance cellular accumulation of lutein from C.v-1068. The lutein accumulation and its biosynthesis pathway genes expression were analyzed under low temperature and high luminosity. We find that both cold and high light stresses can induce lutein biosynthesis in C.v-1068. In addition, during dark to light transition also leads to higher lutein productivity and the maximal lutein content (2.86 mg/g DW) was attained after six days dark-cultivated then exposed to 1000 μ photons m-2 s-1 for two days treatment. Consistently, the transcript levels of lutein biosynthesis genes PSY and ZDS were largely enhanced by cold, high light and dark to light transition treatments. And except PSY and ZDS, 1000 μ photons m-2 s-1 luminosity stress also highly increased expression of PDS as compared with low irradiance condition.

Original languageEnglish (US)
Pages (from-to)151-156
Number of pages6
JournalInternational Agricultural Engineering Journal
Volume26
Issue number3
StatePublished - Sep 2017

Bibliographical note

Funding Information:
This work was supported by grants from the science and technology foundation of Beijing municipal education committee and the opening foundation of Beijing key laboratory of biomass waste resource utilization to WW. We gracefully acknowledge financial support of Scientific Research Project from Facing Characteristic Discipline of Beijing Union University KYDE40201704. WW and YC conceived the research; WW and YC designed the experiments; WW, MC, JL and MD performed the research; WW, WH and YC analyzed data; and WW wrote the paper.

Keywords

  • Cold and luminosity stress
  • Gene expression
  • Lutein
  • Microalgae

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