Enhancement of energy production efficiency from mixed biomass of Chlorella pyrenoidosa and cassava starch through combined hydrogen fermentation and methanogenesis

Ao Xia, Jun Cheng, Lingkan Ding, Richen Lin, Wenlu Song, Junhu Zhou, Kefa Cen

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

To enhance energy production efficiency (EPE) from microalgae Chlorella pyrenoidosa (CP), cassava starch (CS) was mixed with CP to optimise the carbon/nitrogen (C/N) ratio and facilitate efficient dark hydrogen fermentation, followed by photo hydrogen fermentation and methanogenesis. Steam heating with dilute acid was a preferred pre-treatment method to hydrolyze mixed biomass. The maximum dark hydrogen yield of 276.2. mL/g total volatile solids (TVS) from the mixed biomass at C/N molar ratio of 25.3 showed 3.7-fold and 1.8-fold increases, respectively, compared with those from only CP and only CS. The maximum dark hydrogen production rate of 31.96. mL/g. TVS/h from the mixed biomass at the C/N molar ratio of 15.6 showed 3.4-fold and 3.7-fold increases, respectively, compared with those from only CP and only CS. The dark and photo hydrogen yield of 664.2. mL/g. TVS and the methane yield of 126.0. mL/g. TVS corresponded to a total EPE of 67.2%.

Original languageEnglish (US)
Pages (from-to)23-30
Number of pages8
JournalApplied Energy
Volume120
DOIs
StatePublished - May 1 2014
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by the National Natural Science Foundation of China ( 51176163 ), National High Technology R&D Program of China ( 2012AA050101 ), International Sci. & Tech. Cooperation Program of China (2012DFG61770 and 2010DFA72730), National Key Technology R&D Program of China ( 2011BAD14B02 ), Program for New Century Excellent Talents in University (NCET-11-0446), Specialized Research Fund for the Doctoral Program of Higher Education (20110101110021), Science and Technology Project of Guangxi Province (1346011-1).

Keywords

  • Cassava starch
  • Energy production efficiency
  • Hydrogen fermentation
  • Methanogenesis
  • Microalgae

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