Enhanced uptake of dissolved oxygen and glucose by Escherichia coli in a turbulent flow

Amer Al-Homoud, Miki Hondzo

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Laboratory experiments were conducted to study the effect of turbulence on Escherichia coli cells in an oscillating grid reactor under conditions of no oxygen transfer to the liquid phase. Fluid flow was quantified at a submillimeter resolution using a particle image velocimetry measuring technique. The root-mean-square estimates of the velocity gradient tensor components indicated the dominance of shear rate deformation in the fluid surrounding E. coli. The E. coli growth rate, dissolved oxygen (DO), and glucose uptake rates were facilitated by fluid-flow energy dissipation in the turbulent fluid. The Kolmogorov length scale (ηK) and velocity (uK) underlined characteristic scales at which enhanced DO and glucose uptake by E. coli were determined in a turbulent flow in comparison to still-water controls. A first-order power-law relation between the mass transport to the cells and the moving fluid is developed. The combined effects of the enhanced rate of strain at ηK scale and uniform velocity at uK determined the facilitated DO and glucose fluxes to E. coli. The mass transport to the E. coli was modeled by the Sherwood (Sh)-Péclet (Pe) number relationship by Sh = 1 + 1.08 PeuK0.62 where PeuK is the Péclet number defined by the uK velocity scale. The proposed first-order model described experimental data fairly well.

Original languageEnglish (US)
Pages (from-to)643-655
Number of pages13
JournalApplied Microbiology and Biotechnology
Volume79
Issue number4
DOIs
StatePublished - Jun 1 2008

Keywords

  • E. coli
  • Fluid flow
  • Mass transfer
  • Scale up
  • Turbulence

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