Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line

H. S. Kim, J. Kwang, I. J. Yoon, H. S. Joo, M. L. Frey

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497 Scopus citations

Abstract

Two different cell populations, high- (MARC-145) and low-permissive cell clones (L-1) to porcine reproductive and respiratory syndrome (PRRS) virus, were derived from MA-104 cell line (parent cell: P) by cell cloning. Maximum virus yields in MARC-145, P, and L-1 cell clones were 108.5, 103.5, and 102.5 tissue culture infective dose 50 (TCID50)/0.1 ml, respectively. The MARC-145 cell clone supported replication of all 11 different porcine reproductive and respiratory syndrome virus isolates that were tested. These results indicated that the MARC-145 cells will be useful for PRRS virus replication.

Original languageEnglish (US)
Pages (from-to)477-483
Number of pages7
JournalArchives of Virology
Volume133
Issue number3-4
DOIs
StatePublished - Sep 1 1993

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