Enhanced productivity of Protein C by recombinant human cells in automated fed-batch cultures

Yun Seung Kyung, Wei Shou Hu

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Recombinant human kidney cells, 293, were cultivated in serum-free fed-batch cultures for the production of Protein C. By coupling the feeding of concentrated medium to pH control based on established stoichiometric relations, the titer of Protein C increased by more than ten fold as compared to batch culture, even though the total cell concentration increased only by less than two fold. Such a fed-batch culture is a simple system for enhancing the productivity of mammalian cells in culture.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalCytotechnology
Volume17
Issue number2
DOIs
StatePublished - Jan 1995

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