Enhanced Production of κ-Carrageenase and κ-Carrageenan Oligosaccharides through Immobilization of Thalassospira sp. Fjfst-332 with Magnetic Fe3O4-Chitosan Microspheres

Juanjuan Guo, Zhichang Zheng, Chi Chen, Xu Lu, Yi Zhang, Baodong Zheng

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


In this study, immobilized bacteria (IMB) microsphere was prepared by embedding κ-carrageenase-producing Thalassospira sp. Fjfst-332 (TF332) onto a magnetic Fe3O4-chitosan carrier. The performance of Fe3O4-chitosan carrier was optimized by comparing its bacteria immobilization capacity at different Fe3O4:chitosan ratios and temperatures, while the functions of IMB microspheres were characterized by examining their κ-carrageenase production at different temperatures, pH's, and reuse cycles. At the 1:1 (w:w) Fe3O4:chitosan ratio, the Fe3O4-chitosan carriers possessed sufficient anchoring capacity for bacterial immobilization without significant compromise of their magnetism for magnetic separation of IMB from culture media. The spectroscopic analysis of IMB microspheres indicated that the immobilization of TF332 might affect the amide groups in chitosan. Compared to free bacteria, IMB can produce κ-carrageenase at higher temperature, wider pH range, and faster rate. More importantly, the κ-carrageenase-producing activity was sustained for at least seven reuse cycles. The major κ-carrageenan degradation products of IMB-derived κ-carrageenase were the oligosaccharides containing two to six monosaccharide units. Overall, this Fe3O4-chitosan-TF-332 microsphere has the potential to become a stable and reusable platform for large-scale production of κ-carrageenan oligosaccharides.

Original languageEnglish (US)
Pages (from-to)7934-7943
Number of pages10
JournalJournal of agricultural and food chemistry
Issue number36
StatePublished - Sep 13 2017

Bibliographical note

Funding Information:
*E-mail: zyifst@163.com. *E-mail: zbdfst@yeah.net. ORCID Yi Zhang: 0000-0002-6837-4258 Baodong Zheng: 0000-0003-3133-1219 Funding The work was supported by the National Marine Public Welfare Industry Special Scientific Research Projects (No. 201505033) and the Science and Technology Major Project of Fujian Province (No. 2015NZ0001-1). Notes The authors declare no competing financial interest.

Publisher Copyright:
© 2017 American Chemical Society.


  • Thalassospira sp. Fjfst-332
  • immobilized bacteria
  • magnetic FeO-carriers
  • κ-carrageenan oligosaccharide
  • κ-carrageenase


Dive into the research topics of 'Enhanced Production of κ-Carrageenase and κ-Carrageenan Oligosaccharides through Immobilization of Thalassospira sp. Fjfst-332 with Magnetic Fe<sub>3</sub>O<sub>4</sub>-Chitosan Microspheres'. Together they form a unique fingerprint.

Cite this