Enhanced potency of covalently modified granulocyte colony-stimulating factor

Casim Sarkar; Peggy Wang; Douglas Lauffenburger

Enhanced potency of covalently modified granulocyte colony-stimulating factor

Casim Sarkar
, Peggy Wang
, Douglas Lauffenburger

Biomedical Engineering

Research output: Contribution to journal › Conference article › peer-review

Abstract

We have measured the degradation of GCSF and a PEGylated GCSF analog (SD/01) over time in vitro, and both fit a first-order depletion model. We found that SD/01 has a 5-fold longer half-life than GCSF at starting concentrations of 270 pM. However, an increase in the cellular response due to SD/01 was not noticeable until after the fifth day. These results suggest that GCSF and SD/01 may have different trafficking properties.

Original language	English (US)
Pages (from-to)	S-83
Journal	Annals of Biomedical Engineering
Volume	28
Issue number	SUPPL. 1
State	Published - 2000
Event	2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA Duration: Oct 12 2000 → Oct 14 2000

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title = "Enhanced potency of covalently modified granulocyte colony-stimulating factor",

abstract = "We have measured the degradation of GCSF and a PEGylated GCSF analog (SD/01) over time in vitro, and both fit a first-order depletion model. We found that SD/01 has a 5-fold longer half-life than GCSF at starting concentrations of 270 pM. However, an increase in the cellular response due to SD/01 was not noticeable until after the fifth day. These results suggest that GCSF and SD/01 may have different trafficking properties.",

author = "Casim Sarkar and Peggy Wang and Douglas Lauffenburger",

year = "2000",

language = "English (US)",

volume = "28",

pages = "S--83",

journal = "Annals of Biomedical Engineering",

issn = "0090-6964",

publisher = "Springer",

number = "SUPPL. 1",

note = "2000 Annual Fall Meeting of the Biomedical Engineering Society ; Conference date: 12-10-2000 Through 14-10-2000",

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TY - JOUR

T1 - Enhanced potency of covalently modified granulocyte colony-stimulating factor

AU - Sarkar, Casim

AU - Wang, Peggy

AU - Lauffenburger, Douglas

PY - 2000

Y1 - 2000

N2 - We have measured the degradation of GCSF and a PEGylated GCSF analog (SD/01) over time in vitro, and both fit a first-order depletion model. We found that SD/01 has a 5-fold longer half-life than GCSF at starting concentrations of 270 pM. However, an increase in the cellular response due to SD/01 was not noticeable until after the fifth day. These results suggest that GCSF and SD/01 may have different trafficking properties.

AB - We have measured the degradation of GCSF and a PEGylated GCSF analog (SD/01) over time in vitro, and both fit a first-order depletion model. We found that SD/01 has a 5-fold longer half-life than GCSF at starting concentrations of 270 pM. However, an increase in the cellular response due to SD/01 was not noticeable until after the fifth day. These results suggest that GCSF and SD/01 may have different trafficking properties.

UR - https://www.scopus.com/pages/publications/0034498489

UR - https://www.scopus.com/pages/publications/0034498489#tab=citedBy

M3 - Conference article

AN - SCOPUS:0034498489

SN - 0090-6964

VL - 28

SP - S-83

JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

IS - SUPPL. 1

T2 - 2000 Annual Fall Meeting of the Biomedical Engineering Society

Y2 - 12 October 2000 through 14 October 2000

ER -

Enhanced potency of covalently modified granulocyte colony-stimulating factor

Abstract

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