We have measured the degradation of GCSF and a PEGylated GCSF analog (SD/01) over time in vitro, and both fit a first-order depletion model. We found that SD/01 has a 5-fold longer half-life than GCSF at starting concentrations of 270 pM. However, an increase in the cellular response due to SD/01 was not noticeable until after the fifth day. These results suggest that GCSF and SD/01 may have different trafficking properties.
|Original language||English (US)|
|Journal||Annals of Biomedical Engineering|
|Issue number||SUPPL. 1|
|State||Published - Dec 1 2000|
|Event||2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA|
Duration: Oct 12 2000 → Oct 14 2000