Enhanced laccase production by Trametes hirusta using wheat bran under submerged fermentation

S. Bakkiyaraj, R. Aravindan, S. Arrivukkarasan, T. Viruthagiri

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The productivity of laccaseusing white rot fungus Trametes hirsuta was observed in a shaking liquid culture. Gradual increments in laccase production were achieved via screening of the carbon, nitrogen and inducer by one factor at a time method. Time course of laccase production and time course of inducer additionwas monitored.Laccase production of around 9300 U/Lwas obtained with wheat bran as the carbon source, which is one of the lignocellulosic materialsabundantly found as an agro industrial waste, and the production was increased to 17390 U/L with wheat bran and peptone combination. Addition of 1mM 2, 5 xylidinein combination with 1mM copper sulphate on third day enhanced the laccase production to 23543U/L. Further enhancement in laccase production and the influence of significant media components wereaccomplishedusing plakettburmann design (PB) statistical method with the maximum laccase activity of 25889 U/L. The following components viz, wheat bran, peptone, magnesium sulphate, copper sulphate and 2, 5 xylidine were found to be the significant media components.

Original languageEnglish (US)
Pages (from-to)1224-1238
Number of pages15
JournalInternational Journal of ChemTech Research
Volume5
Issue number3
StatePublished - May 28 2013

Fingerprint

Laccase
Fermentation
Copper Sulfate
Peptones
Carbon
Industrial Waste
Copper
Magnesium Sulfate
Industrial wastes
Fungi
Magnesium
Dietary Fiber
Statistical methods
Screening
Nitrogen
Productivity
Liquids
Sulfates

Keywords

  • 2,5 xylidine
  • Laccase
  • Peptone
  • Plakettburmann design
  • Trameteshirsuta
  • Wheat bran

Cite this

Bakkiyaraj, S., Aravindan, R., Arrivukkarasan, S., & Viruthagiri, T. (2013). Enhanced laccase production by Trametes hirusta using wheat bran under submerged fermentation. International Journal of ChemTech Research, 5(3), 1224-1238.

Enhanced laccase production by Trametes hirusta using wheat bran under submerged fermentation. / Bakkiyaraj, S.; Aravindan, R.; Arrivukkarasan, S.; Viruthagiri, T.

In: International Journal of ChemTech Research, Vol. 5, No. 3, 28.05.2013, p. 1224-1238.

Research output: Contribution to journalArticle

Bakkiyaraj, S, Aravindan, R, Arrivukkarasan, S & Viruthagiri, T 2013, 'Enhanced laccase production by Trametes hirusta using wheat bran under submerged fermentation', International Journal of ChemTech Research, vol. 5, no. 3, pp. 1224-1238.
Bakkiyaraj S, Aravindan R, Arrivukkarasan S, Viruthagiri T. Enhanced laccase production by Trametes hirusta using wheat bran under submerged fermentation. International Journal of ChemTech Research. 2013 May 28;5(3):1224-1238.
Bakkiyaraj, S. ; Aravindan, R. ; Arrivukkarasan, S. ; Viruthagiri, T. / Enhanced laccase production by Trametes hirusta using wheat bran under submerged fermentation. In: International Journal of ChemTech Research. 2013 ; Vol. 5, No. 3. pp. 1224-1238.
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