Enhanced laccase production by Trametes hirusta using wheat bran under submerged fermentation

The productivity of laccaseusing white rot fungus Trametes hirsuta was observed in a shaking liquid culture. Gradual increments in laccase production were achieved via screening of the carbon, nitrogen and inducer by one factor at a time method. Time course of laccase production and time course of inducer additionwas monitored.Laccase production of around 9300 U/Lwas obtained with wheat bran as the carbon source, which is one of the lignocellulosic materialsabundantly found as an agro industrial waste, and the production was increased to 17390 U/L with wheat bran and peptone combination. Addition of 1mM 2, 5 xylidinein combination with 1mM copper sulphate on third day enhanced the laccase production to 23543U/L. Further enhancement in laccase production and the influence of significant media components wereaccomplishedusing plakettburmann design (PB) statistical method with the maximum laccase activity of 25889 U/L. The following components viz, wheat bran, peptone, magnesium sulphate, copper sulphate and 2, 5 xylidine were found to be the significant media components.