Enhanced hydrogen production of Enterobacter aerogenes mutated by nuclear irradiation

Jun Cheng, Min Liu, Wenlu Song, Lingkan Ding, Jianzhong Liu, Li Zhang, Kefa Cen

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Nuclear irradiation was used for the first time to generate efficient mutants of hydrogen-producing bacteria Enterobacter aerogenes, which were screened with larger colour circles of more fermentative acid by-products. E. aerogenes cells were mutated by nuclear irradiation of60Co γ-rays. The screened E. aerogenes ZJU1 mutant with larger colour circles enhanced the hydrogenase activity from 89.8 of the wild strain to 157.4 mL H2/(g DW h). The hereditary stability of the E. aerogenes ZJU1 mutant was certified after over ten generations of cultivation. The hydrogen yield of 301 mL H2/g glucose with the mutant was higher by 81.8% than that of 166 mL/g glucose with the wild strain. The peak hydrogen production rate of 27.2 mL/(L·h) with the mutant was higher by 40.9% compared with that of 19.3 mL/(L·h) with the wild strain. The mutant produced more acetate and butyrate but less ethanol compared with the wild strain during hydrogen fermentation.

Original languageEnglish (US)
Pages (from-to)50-55
Number of pages6
JournalBioresource Technology
StatePublished - 2017
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ltd


  • Bacteria mutant
  • Enterobacter aerogenes
  • Hydrogen
  • Nuclear irradiation


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