Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction

G. F. Jones, G. E. Ward, M. P. Murtaugh, G. Lin, C. J. Gebhart

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178 Scopus citations

Abstract

A sensitive assay based on amplification of a 319-bp DNA fragment of the intracellular bacterium of swine proliferative enteritis was developed for the detection of the organism in the feces of swine. A vernacular name, ileal symbiont intracellularis (IS-intracellularis), has recently been published for the intracellular bacterium, which was formerly known as a Campylobacter- like organism (C. J. Gebhart, S. M. Barnes, S. McOrist, G. F. Lin, and G. H. K. Larson, Int. J. Syst. Bacteriol. 43:533-538, 1993). As few as 101 IS- intracellularis organisms purified from intestinal mucosa, or 103 IS- intracellularis per g of feces, were detected. No amplification product was produced from a polymerase chain reaction performed on DNA extracted from the feces of healthy pigs. A 319-bp DNA fragment specific for IS-intracellularis was produced on amplification of DNA from the feces of pigs with experimental and naturally occurring proliferative enteritis.

Original languageEnglish (US)
Pages (from-to)2611-2615
Number of pages5
JournalJournal of clinical microbiology
Volume31
Issue number10
DOIs
StatePublished - 1993

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