TY - JOUR
T1 - Enhanced dark hydrogen fermentation by addition of ferric oxide nanoparticles using Enterobacter aerogenes
AU - Lin, Richen
AU - Cheng, Jun
AU - Ding, Lingkan
AU - Song, Wenlu
AU - Liu, Min
AU - Zhou, Junhu
AU - Cen, Kefa
N1 - Publisher Copyright:
© 2016 Elsevier Ltd.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Ferric oxide nanoparticles (FONPs) were used to facilitate dark hydrogen fermentation using Enterobacter aerogenes. The hydrogen yield of glucose increased from 164.5 ± 2.29 to 192.4 ± 1.14 mL/g when FONPs concentration increased from 0 to 200 mg/L. SEM images of E. aerogenes demonstrated the existence of bacterial nanowire among cells, suggesting FONPs served as electron conduits to enhance electron transfer. TEM showed cellular internalization of FONPs, indicating hydrogenase synthesis and activity was potentially promoted due to the released iron element. When further increasing FONPs concentration to 400 mg/L, the hydrogen yield of glucose decreased to 147.2 ± 2.54 mL/g. Soluble metabolic products revealed FONPs enhanced acetate pathway of hydrogen production, but weakened ethanol pathway. This shift of metabolic pathways allowed more nicotinamide adenine dinucleotide for reducing proton to hydrogen.
AB - Ferric oxide nanoparticles (FONPs) were used to facilitate dark hydrogen fermentation using Enterobacter aerogenes. The hydrogen yield of glucose increased from 164.5 ± 2.29 to 192.4 ± 1.14 mL/g when FONPs concentration increased from 0 to 200 mg/L. SEM images of E. aerogenes demonstrated the existence of bacterial nanowire among cells, suggesting FONPs served as electron conduits to enhance electron transfer. TEM showed cellular internalization of FONPs, indicating hydrogenase synthesis and activity was potentially promoted due to the released iron element. When further increasing FONPs concentration to 400 mg/L, the hydrogen yield of glucose decreased to 147.2 ± 2.54 mL/g. Soluble metabolic products revealed FONPs enhanced acetate pathway of hydrogen production, but weakened ethanol pathway. This shift of metabolic pathways allowed more nicotinamide adenine dinucleotide for reducing proton to hydrogen.
KW - Enterobacter aerogenes
KW - Ferric oxide nanoparticles
KW - Hydrogen fermentation
UR - http://www.scopus.com/inward/record.url?scp=84957962617&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84957962617&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2016.02.009
DO - 10.1016/j.biortech.2016.02.009
M3 - Article
C2 - 26890796
AN - SCOPUS:84957962617
SN - 0960-8524
VL - 207
SP - 213
EP - 219
JO - Bioresource Technology
JF - Bioresource Technology
ER -