Abstract
Membrane-engineered cells displaying antigen-targeting ligands are useful as both scientific tools and clinical therapeutics. While genetically encoded artificial receptors have proven efficacious, their scope remains limited, as this approach is not amenable to all cell types and the modification is often permanent. Our group has developed a nongenetic method to rapidly, stably, and reversibly modify any cell membrane with a chemically self-assembled nanoring (CSAN) that can function as a prosthetic receptor. Bifunctional CSANs displaying epithelial cell adhesion molecule (EpCAM)-targeted fibronectin domains were installed on the cell membrane through hydrophobic insertion and remained stably bound for ≥72 h in vitro. These CSAN-labeled cells were capable of recognizing EpCAM-expressing target cells, forming intercellular interactions that were subsequently reversed by disassembling the nanoring with the FDA-approved antibiotic, trimethoprim. This study demonstrates the use of this system to engineer cell surfaces with prosthetic receptors capable of directing specific and reversible cell-cell interactions.
Original language | English (US) |
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Pages (from-to) | 1291-1301 |
Number of pages | 11 |
Journal | Bioconjugate Chemistry |
Volume | 29 |
Issue number | 4 |
DOIs | |
State | Published - Apr 18 2018 |
Bibliographical note
Funding Information:This work was supported by the National Institutes of Health R21 CA185627 (CRW), F30 CA210345 (CMC), T32 GM008244 (CMC), R21 EB019518 (BJH), and the University of Minnesota. Parts of this work were carried out in the Characterization Facility, University of Minnesota, which receives partial support from NSF through the MRSEC program. The authors gratefully acknowledge Dr. Bob Hafner (Characterization Facility, University of Minnesota) for his assistance with the transmission electron microscopy experiments. Microscopy work was performed in the University Imaging Center at the University of Minnesota.
Funding Information:
This work was supported by the National Institutes of Health R21 CA185627 (CRW), F30 CA210345 (CMC), T32 GM008244 (CMC), R21 EB019518 (BJH), and the University of Minnesota. Parts of this work were carried out in the Characterization Facility, University of Minnesota, which receives partial support from NSF through the MRSEC program. The authors gratefully acknowledge Dr. Bob Hafner (Characterization Facility University of Minnesota) for his assistance with the transmission electron microscopy experiments. Microscopy work was performed in the University Imaging Center at the University of Minnesota.
Publisher Copyright:
© 2018 American Chemical Society.
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Sanders, M. A. (Program Director)
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