Techniques to direct cell-cell interactions have advanced our understanding of fundamental biology and opened new avenues in tissue engineering, regenerative medicine, and immunotherapy. This is often achieved by introducing new targeting ligands to the cell membrane, which can be accomplished through both genetic and nongenetic approaches. While both offer advantages, nongenetic modifications tend to be faster to produce, innocuous to the modified cell, and potentially reversible. This chapter will outline nongenetic methods that have been used to control intercellular interactions—namely hydrophobic insertion, chemical modification, liposome fusion, metabolic engineering, and enzymatic remodeling—and provide protocols that can serve as a starting point for future applications.
|Original language||English (US)|
|Title of host publication||Methods in Enzymology|
|Editors||David M. Chenoweth|
|Publisher||Academic Press Inc.|
|Number of pages||24|
|State||Published - 2020|
|Name||Methods in Enzymology|
Bibliographical noteFunding Information:
The authors gratefully acknowledge support from the National Institutes of Health R21 CA185627 (C.R.W.), T32 GM008244 (C.M.C.), and the University of Minnesota Foundation.
© 2020 Elsevier Inc.
Copyright 2020 Elsevier B.V., All rights reserved.
- Cellular immunotherapy
- Hydrophobic insertion
- Intercellular interactions
- Liposome fusion
- Membrane engineering
- Metabolic engineering
- Regenerative medicine
- Tissue engineering
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't
- Research Support, N.I.H., Extramural