Engineering of streptomyces platensis MA7339 for overproduction of platencin and congeners

Zhiguo Yu; Michael J. Smanski; Ryan M. Peterson; Karen Marchillo; David Andes; Scott R. Rajski; Ben Shen

doi:10.1021/ol100342m

Engineering of streptomyces platensis MA7339 for overproduction of platencin and congeners

Zhiguo Yu, Michael J. Smanski, Ryan M. Peterson, Karen Marchillo, David Andes, Scott R. Rajski, Ben Shen

Biochemistry, Molecular Biology, and Biophysics (CBS)

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Abstract

Platensimycin (1) and platencin (2) are novel antibiotic leads against multidrug resistant pathogens. The production of 2 in Streptomyces platensis MA7339 is under the control of ptnR1, a GntR-like transcriptional regulator. Inactivating ptnR1 afforded S. platensis MA7339 mutant strain SB12600 that overproduces 2 at a titer -100-fold greater than that from the wild-type strain and accumulates platencin A₁ (3) and eight new congeners, platencins A₂-A₉ (4-11). The isolation, structural elucidation, and antibacterial activity of 4-11, in comparison to 1-3, are described.

Original language	English (US)
Pages (from-to)	1744-1747
Number of pages	4
Journal	Organic Letters
Volume	12
Issue number	8
DOIs	https://doi.org/10.1021/ol100342m
State	Published - Apr 16 2010

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10.1021/ol100342m

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title = "Engineering of streptomyces platensis MA7339 for overproduction of platencin and congeners",

abstract = "Platensimycin (1) and platencin (2) are novel antibiotic leads against multidrug resistant pathogens. The production of 2 in Streptomyces platensis MA7339 is under the control of ptnR1, a GntR-like transcriptional regulator. Inactivating ptnR1 afforded S. platensis MA7339 mutant strain SB12600 that overproduces 2 at a titer -100-fold greater than that from the wild-type strain and accumulates platencin A1 (3) and eight new congeners, platencins A2-A9 (4-11). The isolation, structural elucidation, and antibacterial activity of 4-11, in comparison to 1-3, are described.",

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T1 - Engineering of streptomyces platensis MA7339 for overproduction of platencin and congeners

AU - Yu, Zhiguo

AU - Smanski, Michael J.

AU - Peterson, Ryan M.

AU - Marchillo, Karen

AU - Andes, David

AU - Rajski, Scott R.

AU - Shen, Ben

PY - 2010/4/16

Y1 - 2010/4/16

N2 - Platensimycin (1) and platencin (2) are novel antibiotic leads against multidrug resistant pathogens. The production of 2 in Streptomyces platensis MA7339 is under the control of ptnR1, a GntR-like transcriptional regulator. Inactivating ptnR1 afforded S. platensis MA7339 mutant strain SB12600 that overproduces 2 at a titer -100-fold greater than that from the wild-type strain and accumulates platencin A1 (3) and eight new congeners, platencins A2-A9 (4-11). The isolation, structural elucidation, and antibacterial activity of 4-11, in comparison to 1-3, are described.

AB - Platensimycin (1) and platencin (2) are novel antibiotic leads against multidrug resistant pathogens. The production of 2 in Streptomyces platensis MA7339 is under the control of ptnR1, a GntR-like transcriptional regulator. Inactivating ptnR1 afforded S. platensis MA7339 mutant strain SB12600 that overproduces 2 at a titer -100-fold greater than that from the wild-type strain and accumulates platencin A1 (3) and eight new congeners, platencins A2-A9 (4-11). The isolation, structural elucidation, and antibacterial activity of 4-11, in comparison to 1-3, are described.

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Engineering of streptomyces platensis MA7339 for overproduction of platencin and congeners

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