TY - JOUR
T1 - Engineering nonphosphorylative metabolism to generate lignocellulose-derived products
AU - Tai, Yi Shu
AU - Xiong, Mingyong
AU - Jambunathan, Pooja
AU - Wang, Jingyu
AU - Wang, Jilong
AU - Stapleton, Cole
AU - Zhang, Kechun
N1 - Publisher Copyright:
© 2016 Nature America, Inc. All rights reserved.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Conversion of lignocellulosic biomass into value-added products provides important environmental and economic benefits. Here we report the engineering of an unconventional metabolism for the production of tricarboxylic acid (TCA)-cycle derivatives from D-xylose, L-arabinose and D-galacturonate. We designed a growth-based selection platform to identify several gene clusters functional in Escherichia coli that can perform this nonphosphorylative assimilation of sugars into the TCA cycle in less than six steps. To demonstrate the application of this new metabolic platform, we built artificial biosynthetic pathways to 1,4-butanediol (BDO) with a theoretical molar yield of 100%. By screening and engineering downstream pathway enzymes, 2-ketoacid decarboxylases and alcohol dehydrogenases, we constructed E. coli strains capable of producing BDO from D-xylose, L-arabinose and D-galacturonate. The titers, rates and yields were higher than those previously reported using conventional pathways. This work demonstrates the potential of nonphosphorylative metabolism for biomanufacturing with improved biosynthetic efficiencies.
AB - Conversion of lignocellulosic biomass into value-added products provides important environmental and economic benefits. Here we report the engineering of an unconventional metabolism for the production of tricarboxylic acid (TCA)-cycle derivatives from D-xylose, L-arabinose and D-galacturonate. We designed a growth-based selection platform to identify several gene clusters functional in Escherichia coli that can perform this nonphosphorylative assimilation of sugars into the TCA cycle in less than six steps. To demonstrate the application of this new metabolic platform, we built artificial biosynthetic pathways to 1,4-butanediol (BDO) with a theoretical molar yield of 100%. By screening and engineering downstream pathway enzymes, 2-ketoacid decarboxylases and alcohol dehydrogenases, we constructed E. coli strains capable of producing BDO from D-xylose, L-arabinose and D-galacturonate. The titers, rates and yields were higher than those previously reported using conventional pathways. This work demonstrates the potential of nonphosphorylative metabolism for biomanufacturing with improved biosynthetic efficiencies.
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U2 - 10.1038/nchembio.2020
DO - 10.1038/nchembio.2020
M3 - Article
C2 - 26854668
AN - SCOPUS:84957622111
VL - 12
SP - 247
EP - 253
JO - Nature Chemical Biology
JF - Nature Chemical Biology
SN - 1552-4450
IS - 4
ER -