Engineering nonphosphorylative metabolism to generate lignocellulose-derived products

Yi Shu Tai, Mingyong Xiong, Pooja Jambunathan, Jingyu Wang, Jilong Wang, Cole Stapleton, Kechun Zhang

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Conversion of lignocellulosic biomass into value-added products provides important environmental and economic benefits. Here we report the engineering of an unconventional metabolism for the production of tricarboxylic acid (TCA)-cycle derivatives from D-xylose, L-arabinose and D-galacturonate. We designed a growth-based selection platform to identify several gene clusters functional in Escherichia coli that can perform this nonphosphorylative assimilation of sugars into the TCA cycle in less than six steps. To demonstrate the application of this new metabolic platform, we built artificial biosynthetic pathways to 1,4-butanediol (BDO) with a theoretical molar yield of 100%. By screening and engineering downstream pathway enzymes, 2-ketoacid decarboxylases and alcohol dehydrogenases, we constructed E. coli strains capable of producing BDO from D-xylose, L-arabinose and D-galacturonate. The titers, rates and yields were higher than those previously reported using conventional pathways. This work demonstrates the potential of nonphosphorylative metabolism for biomanufacturing with improved biosynthetic efficiencies.

Original languageEnglish (US)
Pages (from-to)247-253
Number of pages7
JournalNature Chemical Biology
Volume12
Issue number4
DOIs
StatePublished - Apr 1 2016

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Arabinose
Citric Acid Cycle
Xylose
Escherichia coli
Carboxy-Lyases
Alcohol Dehydrogenase
Biosynthetic Pathways
Multigene Family
Biomass
Economics
Enzymes
Growth
lignocellulose
1,4-butanediol

Cite this

Tai, Y. S., Xiong, M., Jambunathan, P., Wang, J., Wang, J., Stapleton, C., & Zhang, K. (2016). Engineering nonphosphorylative metabolism to generate lignocellulose-derived products. Nature Chemical Biology, 12(4), 247-253. https://doi.org/10.1038/nchembio.2020

Engineering nonphosphorylative metabolism to generate lignocellulose-derived products. / Tai, Yi Shu; Xiong, Mingyong; Jambunathan, Pooja; Wang, Jingyu; Wang, Jilong; Stapleton, Cole; Zhang, Kechun.

In: Nature Chemical Biology, Vol. 12, No. 4, 01.04.2016, p. 247-253.

Research output: Contribution to journalArticle

Tai, YS, Xiong, M, Jambunathan, P, Wang, J, Wang, J, Stapleton, C & Zhang, K 2016, 'Engineering nonphosphorylative metabolism to generate lignocellulose-derived products', Nature Chemical Biology, vol. 12, no. 4, pp. 247-253. https://doi.org/10.1038/nchembio.2020
Tai YS, Xiong M, Jambunathan P, Wang J, Wang J, Stapleton C et al. Engineering nonphosphorylative metabolism to generate lignocellulose-derived products. Nature Chemical Biology. 2016 Apr 1;12(4):247-253. https://doi.org/10.1038/nchembio.2020
Tai, Yi Shu ; Xiong, Mingyong ; Jambunathan, Pooja ; Wang, Jingyu ; Wang, Jilong ; Stapleton, Cole ; Zhang, Kechun. / Engineering nonphosphorylative metabolism to generate lignocellulose-derived products. In: Nature Chemical Biology. 2016 ; Vol. 12, No. 4. pp. 247-253.
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