Engineering meganuclease for precise plant genome modification

Fayza Daboussi, Thomas J. Stoddard, Feng Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Meganucleases, also termed homing endonucleases, are rare-cutting enzymes that are encoded within the genome of nearly all microbes. These enzymes recognize and cleave long DNA sequences (typically 18–30 base pairs) generating double-strand DNA breaks (DSBs). The resulting DSBs can be repaired by different pathways leading to a variety of site-specifi c DNA modifi cations, such as insertions, deletions, or point mutations. Over the past 15 years tremendous efforts have been made to engineer a number of variant meganucleases that cleave novel DNA targets. Engineered meganucleases are now being used to generate targeted genomic modifi cations for a variety of basic and biotechnology applications, including creating valuable traits in crop species.

Original languageEnglish (US)
Title of host publicationAdvances in New Technology for Targeted Modification of Plant Genomes
PublisherSpringer New York
Pages21-38
Number of pages18
ISBN (Electronic)9781493925568
ISBN (Print)9781493925551
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Keywords

  • Homologous gene targeting
  • Meganuclease
  • Precise genome engineering
  • Sequence-specific nucleases
  • Targeted mutagenesis

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  • Cite this

    Daboussi, F., Stoddard, T. J., & Zhang, F. (2015). Engineering meganuclease for precise plant genome modification. In Advances in New Technology for Targeted Modification of Plant Genomes (pp. 21-38). Springer New York. https://doi.org/10.1007/978-1-4939-2556-8_2