Abstract
Agrobacterium rhizogenes is a well characterized bacterium for “hairy root induction” due to presence of Ri plasmid. Ri plasmid has been modified and engineered with required foreign genes and used as a binary vector for plant genetic transformation. A. rhizogenes-mediated hairy root induction and cultures of recalcitrant plant species are useful in genetic and metabolic engineering for secondary metabolite and recombinant protein production. With the advancement of CRISPR/Cas9 genome editing tools, plant genome can be easily manipulated for metabolic engineering. However, CRISPR/Cas9-mediated genome editing requires efficient A. rhizogenes-mediated genetic transformation and selection. In this chapter, we discussed the different essential component of CRISPR/Cas9 editing tools. Different types of CRISPR/Cas9 vectors are now available for various purposes such as disruption, replacement, transcriptional activators, and inhibitors of desired gene.
| Original language | English (US) |
|---|---|
| Title of host publication | Hairy Roots |
| Subtitle of host publication | An Effective Tool of Plant Biotechnology |
| Publisher | Springer Singapore |
| Pages | 329-342 |
| Number of pages | 14 |
| ISBN (Electronic) | 9789811325625 |
| ISBN (Print) | 9789811325618 |
| DOIs | |
| State | Published - Jan 1 2018 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© Springer Nature Singapore Pte Ltd. 2018.
Keywords
- Agrobacterium rhizogenes
- CRISPR/Cas9
- Genome editing
- Hairy root
- Transformation