Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis

Miller Huang, Jignesh Tailor, Qiqi Zhen, Aaron H. Gillmor, Matthew L. Miller, Holger Weishaupt, Justin Chen, Tina Zheng, Emily K. Nash, Lauren K. McHenry, Zhenyi An, Fubaiyang Ye, Yasuhiro Takashima, James Clarke, Harold Ayetey, Florence M.G. Cavalli, Betty Luu, Branden S. Moriarity, Shirin Ilkhanizadeh, Lukas ChavezChunying Yu, Kathreena M. Kurian, Thierry Magnaldo, Nicolas Sevenet, Philipp Koch, Steven M. Pollard, Peter Dirks, Michael P. Snyder, David A. Largaespada, Yoon Jae Cho, Joanna J. Phillips, Fredrik J. Swartling, A. Sorana Morrissy, Marcel Kool, Stefan M. Pfister, Michael D. Taylor, Austin Smith, William A. Weiss

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Huang, Tailor, et al. show that neuroepithelial stem (NES) cells derived from normal induced pluripotent stem cells (iPSCs) provide a renewable human cell-based resource to evaluate genetic mutations in medulloblastoma. Misexpression of MYCN in both otherwise-normal NES cells and PTCH1+/− NES cells derived from patients with Gorlin syndrome each generated medulloblastoma in mice.

Original languageEnglish (US)
Pages (from-to)433-446.e7
JournalCell Stem Cell
Volume25
Issue number3
DOIs
StatePublished - Sep 5 2019

Keywords

  • SHH
  • human pluripotent stem cells
  • medulloblastoma
  • neuroepithelial stem cells

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    Huang, M., Tailor, J., Zhen, Q., Gillmor, A. H., Miller, M. L., Weishaupt, H., Chen, J., Zheng, T., Nash, E. K., McHenry, L. K., An, Z., Ye, F., Takashima, Y., Clarke, J., Ayetey, H., Cavalli, F. M. G., Luu, B., Moriarity, B. S., Ilkhanizadeh, S., ... Weiss, W. A. (2019). Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis. Cell Stem Cell, 25(3), 433-446.e7. https://doi.org/10.1016/j.stem.2019.05.013