Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis

Miller Huang; Jignesh Tailor; Qiqi Zhen; Aaron H. Gillmor; Matthew L. Miller; Holger Weishaupt; Justin Chen; Tina Zheng; Emily K. Nash; Lauren K. McHenry; Zhenyi An; Fubaiyang Ye; Yasuhiro Takashima; James Clarke; Harold Ayetey; Florence M.G. Cavalli; Betty Luu; Branden S. Moriarity; Shirin Ilkhanizadeh; Lukas Chavez; Chunying Yu; Kathreena M. Kurian; Thierry Magnaldo; Nicolas Sevenet; Philipp Koch; Steven M. Pollard; Peter Dirks; Michael P. Snyder; David A. Largaespada; Yoon Jae Cho; Joanna J. Phillips; Fredrik J. Swartling; A. Sorana Morrissy; Marcel Kool; Stefan M. Pfister; Michael D. Taylor; Austin Smith; William A. Weiss

doi:10.1016/j.stem.2019.05.013

Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis

Miller Huang, Jignesh Tailor, Qiqi Zhen, Aaron H. Gillmor, Matthew L. Miller, Holger Weishaupt, Justin Chen, Tina Zheng, Emily K. Nash, Lauren K. McHenry, Zhenyi An, Fubaiyang Ye, Yasuhiro Takashima, James Clarke, Harold Ayetey, Florence M.G. Cavalli, Betty Luu, Branden S. Moriarity, Shirin Ilkhanizadeh, Lukas ChavezChunying Yu, Kathreena M. Kurian, Thierry Magnaldo, Nicolas Sevenet, Philipp Koch, Steven M. Pollard, Peter Dirks, Michael P. Snyder, David A. Largaespada, Yoon Jae Cho, Joanna J. Phillips, Fredrik J. Swartling, A. Sorana Morrissy, Marcel Kool, Stefan M. Pfister, Michael D. Taylor, Austin Smith, William A. Weiss

Pediatrics - Hematology

Research output: Contribution to journal › Article

12 Scopus citations

Abstract

Huang, Tailor, et al. show that neuroepithelial stem (NES) cells derived from normal induced pluripotent stem cells (iPSCs) provide a renewable human cell-based resource to evaluate genetic mutations in medulloblastoma. Misexpression of MYCN in both otherwise-normal NES cells and PTCH1^+/− NES cells derived from patients with Gorlin syndrome each generated medulloblastoma in mice.

Original language	English (US)
Pages (from-to)	433-446.e7
Journal	Cell Stem Cell
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/j.stem.2019.05.013
State	Published - Sep 5 2019

Keywords

SHH
human pluripotent stem cells
medulloblastoma
neuroepithelial stem cells

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Huang, M., Tailor, J., Zhen, Q., Gillmor, A. H., Miller, M. L., Weishaupt, H., Chen, J., Zheng, T., Nash, E. K., McHenry, L. K., An, Z., Ye, F., Takashima, Y., Clarke, J., Ayetey, H., Cavalli, F. M. G., Luu, B., Moriarity, B. S., Ilkhanizadeh, S., ... Weiss, W. A. (2019). Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis. Cell Stem Cell, 25(3), 433-446.e7. https://doi.org/10.1016/j.stem.2019.05.013

Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis. / Huang, Miller; Tailor, Jignesh; Zhen, Qiqi; Gillmor, Aaron H.; Miller, Matthew L.; Weishaupt, Holger; Chen, Justin; Zheng, Tina; Nash, Emily K.; McHenry, Lauren K.; An, Zhenyi; Ye, Fubaiyang; Takashima, Yasuhiro; Clarke, James; Ayetey, Harold; Cavalli, Florence M.G.; Luu, Betty; Moriarity, Branden S.; Ilkhanizadeh, Shirin; Chavez, Lukas; Yu, Chunying; Kurian, Kathreena M.; Magnaldo, Thierry; Sevenet, Nicolas; Koch, Philipp; Pollard, Steven M.; Dirks, Peter; Snyder, Michael P.; Largaespada, David A.; Cho, Yoon Jae; Phillips, Joanna J.; Swartling, Fredrik J.; Morrissy, A. Sorana; Kool, Marcel; Pfister, Stefan M.; Taylor, Michael D.; Smith, Austin; Weiss, William A.

In: Cell Stem Cell, Vol. 25, No. 3, 05.09.2019, p. 433-446.e7.

Research output: Contribution to journal › Article

Huang, M, Tailor, J, Zhen, Q, Gillmor, AH, Miller, ML, Weishaupt, H, Chen, J, Zheng, T, Nash, EK, McHenry, LK, An, Z, Ye, F, Takashima, Y, Clarke, J, Ayetey, H, Cavalli, FMG, Luu, B, Moriarity, BS, Ilkhanizadeh, S, Chavez, L, Yu, C, Kurian, KM, Magnaldo, T, Sevenet, N, Koch, P, Pollard, SM, Dirks, P, Snyder, MP, Largaespada, DA, Cho, YJ, Phillips, JJ, Swartling, FJ, Morrissy, AS, Kool, M, Pfister, SM, Taylor, MD, Smith, A & Weiss, WA 2019, 'Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis', Cell Stem Cell, vol. 25, no. 3, pp. 433-446.e7. https://doi.org/10.1016/j.stem.2019.05.013

Huang, Miller ; Tailor, Jignesh ; Zhen, Qiqi ; Gillmor, Aaron H. ; Miller, Matthew L. ; Weishaupt, Holger ; Chen, Justin ; Zheng, Tina ; Nash, Emily K. ; McHenry, Lauren K. ; An, Zhenyi ; Ye, Fubaiyang ; Takashima, Yasuhiro ; Clarke, James ; Ayetey, Harold ; Cavalli, Florence M.G. ; Luu, Betty ; Moriarity, Branden S. ; Ilkhanizadeh, Shirin ; Chavez, Lukas ; Yu, Chunying ; Kurian, Kathreena M. ; Magnaldo, Thierry ; Sevenet, Nicolas ; Koch, Philipp ; Pollard, Steven M. ; Dirks, Peter ; Snyder, Michael P. ; Largaespada, David A. ; Cho, Yoon Jae ; Phillips, Joanna J. ; Swartling, Fredrik J. ; Morrissy, A. Sorana ; Kool, Marcel ; Pfister, Stefan M. ; Taylor, Michael D. ; Smith, Austin ; Weiss, William A. / Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis. In: Cell Stem Cell. 2019 ; Vol. 25, No. 3. pp. 433-446.e7.

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abstract = "Huang, Tailor, et al. show that neuroepithelial stem (NES) cells derived from normal induced pluripotent stem cells (iPSCs) provide a renewable human cell-based resource to evaluate genetic mutations in medulloblastoma. Misexpression of MYCN in both otherwise-normal NES cells and PTCH1+/− NES cells derived from patients with Gorlin syndrome each generated medulloblastoma in mice.",

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AU - Weishaupt, Holger

AU - Chen, Justin

AU - Zheng, Tina

AU - Nash, Emily K.

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AU - Ye, Fubaiyang

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AU - Ayetey, Harold

AU - Cavalli, Florence M.G.

AU - Luu, Betty

AU - Moriarity, Branden S.

AU - Ilkhanizadeh, Shirin

AU - Chavez, Lukas

AU - Yu, Chunying

AU - Kurian, Kathreena M.

AU - Magnaldo, Thierry

AU - Sevenet, Nicolas

AU - Koch, Philipp

AU - Pollard, Steven M.

AU - Dirks, Peter

AU - Snyder, Michael P.

AU - Largaespada, David A.

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AU - Phillips, Joanna J.

AU - Swartling, Fredrik J.

AU - Morrissy, A. Sorana

AU - Kool, Marcel

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AU - Smith, Austin

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