Engineering fibronectin-based binding proteins by yeast surface display

Tiffany F. Chen, Seymour De Picciotto, Benjamin J. Hackel, K. Dane Wittrup

Research output: Chapter in Book/Report/Conference proceedingChapter

30 Scopus citations

Abstract

Yeast surface display (YSD) presents proteins on the surface of yeast through interaction of the agglutinin subunits Aga1p and Aga2p. The human 10th type III fibronectin (Fn3) is a small, 10-kDa protein domain that maintains its native fold without disulfide bonds. A YSD library of Fn3s has been engineered with a loop amino acid composition similar to that of human antibody complementarity-determining region heavy chain loop 3 (CDR-H3) and varying loop lengths, which has been shown to improve binding ability. There are many advantages of using these small, stable domains that maintain binding capabilities similar to that of antibodies. Here, we outline a YSD methodology to isolate Fn3 binders to a diverse set of target antigens.

Original languageEnglish (US)
Title of host publicationMethods in Protein Design
PublisherAcademic Press Inc.
Pages303-326
Number of pages24
ISBN (Print)9780123942920
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume523
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Alternative scaffolds
  • Directed evolution
  • Fibronectin type III domain
  • Protein engineering
  • Yeast surface display

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    Chen, T. F., De Picciotto, S., Hackel, B. J., & Wittrup, K. D. (2013). Engineering fibronectin-based binding proteins by yeast surface display. In Methods in Protein Design (pp. 303-326). (Methods in Enzymology; Vol. 523). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-394292-0.00014-X