Engineering cells for cell culture bioprocessing - Physiological fundamentals

Gargi Seth, Patrick Hossler, Chong Yee Joon, Wei Shou Hu

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

In the past decade, we have witnessed a tremendous increase in the number of mammalian cell-derived therapeutic proteins with clinical applications. The success of making these life-saving biologics available to the public is partly due to engineering efforts to enhance process efficiency. To further improve productivity, much effort has been devoted to developing metabolically engineered producing cells, which possess characteristics favorable for large-scale bioprocessing. In this article we discuss the fundamental physiological basis for cell engineering. Different facets of cellular mechanisms, including metabolism, protein processing, and the balancing pathways of cell growth and apoptosis, contribute to the complex traits of favorable growth and production characteristics. We present our assessment of the current state of the art by surveying efforts that have already been undertaken in engineering cells for a more robust process. The concept of physiological homeostasis as a key determinant and its implications on cell engineering is emphasized. Integrating the physiological perspective with cell culture engineering will facilitate attainment of dream cells with superlative characteristics.

Original languageEnglish (US)
Pages (from-to)119-164
Number of pages46
JournalAdvances in Biochemical Engineering/Biotechnology
Volume101
DOIs
StatePublished - Jun 8 2006

Keywords

  • Apoptosis
  • Cell cycle
  • Cell engineering
  • Cell physiology
  • Glycosylation
  • Mammalian cell culture
  • Metabolic engineering
  • Metabolism
  • Protein processing
  • Recombinant protein

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Review

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