Engineering bioscaffolds for enzyme assembly

Hao Dong, Wenxue Zhang, Shengmin Zhou, Jiaofang Huang, Ping Wang

Research output: Contribution to journalReview articlepeer-review

5 Scopus citations


With the demand for green, safe, and continuous biocatalysis, bioscaffolds, compared with synthetic scaffolds, have become a desirable candidate for constructing enzyme assemblages because of their biocompatibility and regenerability. Biocompatibility makes bioscaffolds more suitable for safe and green production, especially in food processing, production of bioactive agents, and diagnosis. The regenerability can enable the engineered biocatalysts regenerate through simple self-proliferation without complex re-modification, which is attractive for continuous biocatalytic processes. In view of the unique biocompatibility and regenerability of bioscaffolds, they can be classified into non-living (polysaccharide, nucleic acid, and protein) and living (virus, bacteria, fungi, spore, and biofilm) bioscaffolds, which can fully satisfy these two unique properties, respectively. Enzymes assembled onto non-living bioscaffolds are based on single or complex components, while enzymes assembled onto living bioscaffolds are based on living bodies. In terms of their unique biocompatibility and regenerability, this review mainly covers the current advances in the research and application of non-living and living bioscaffolds with focus on engineering strategies for enzyme assembly. Finally, the future development of bioscaffolds for enzyme assembly is also discussed. Hopefully, this review will attract the interest of researchers in various fields and empower the development of biocatalysis, biomedicine, environmental remediation, therapy, and diagnosis.

Original languageEnglish (US)
Article number107721
JournalBiotechnology Advances
Early online dateFeb 23 2021
StatePublished - Dec 2021

Bibliographical note

Funding Information:
This work was sponsored by the National Natural Science Foundation of China (21636003, 21672065, 22077032, and 31872728), the International S&T Innovation Cooperation Key Project (2017YFE0129600), the National Science and Technology Major Project of the Ministry of Science and Technology of China (2020YFA0908900), the Natural Science Foundation of Shanghai (19ZR1477100), the Fundamental Research Funds for the Central Universities (22221818014), and the 111 Project (B18022).

Publisher Copyright:
© 2021

Copyright 2021 Elsevier B.V., All rights reserved.


  • Biocompatibility
  • Enzyme assembly
  • Living bioscaffold
  • Non-living bioscaffold
  • Regenerability

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Review


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