Abstract
Enzymes able to degrade or modify acyl-homoserine lactones (AHLs) have drawn considerable interest for their ability to interfere with the bacterial communication process referred to as quorum sensing. Many proteobacteria use AHL to coordinate virulence and biofilm formation in a cell density-dependent manner; thus, AHL-interfering enzymes constitute new promising antimicrobial candidates. Among these, lactonases and acylases have been particularly studied. These enzymes have been isolated from various bacterial, archaeal, or eukaryotic organisms and have been evaluated for their ability to control several pathogens. Engineering studies on these enzymes were carried out and successfully modulated their capacity to interact with specific AHL, increase their catalytic activity and stability, or enhance their biotechnological potential. In this review, special attention is paid to the screening, engineering, and applications of AHL-modifying enzymes. Prospects and future opportunities are also discussed with a view to developing potent candidates for bacterial control.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 12993-13007 |
| Number of pages | 15 |
| Journal | Journal of Biological Chemistry |
| Volume | 295 |
| Issue number | 37 |
| DOIs | |
| State | Published - Sep 11 2020 |
Bibliographical note
Funding Information:his work was supported by the ?Investissements d'avenir? program (M?diterran?e Infection Grant 10-IAHU-03) of the French Agence Nationale de la Recherche (ANR) and RESSAC (Grant ANR-17-ASTR-0003) from the French Direction G?n?rale de l'Armement (DGA). R. B. is a Ph.D. student supported by ANRT CIFRE Grant 2018/1543. M. E. is supported by NIGMS, National Institutes of Health, Grant R35GM133487. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Funding Information:
Funding and additional information—This work was supported by the “Investissements d’avenir” program (Méditerranée Infection Grant 10-IAHU-03) of the French Agence Nationale de la Recherche (ANR) and RESSAC (Grant ANR-17-ASTR-0003) from the French Direction Générale de l’Armement (DGA). R. B. is a Ph.D. student supported by ANRT CIFRE Grant 2018/1543. M. E. is supported by NIGMS, National Institutes of Health, Grant R35GM133487. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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