TY - JOUR
T1 - Engineered swine models of cancer
AU - Watson, Adrienne L.
AU - Carlson, Daniel F.
AU - Largaespada, David A.
AU - Hackett, Perry B.
AU - Fahrenkrug, Scott C.
N1 - Publisher Copyright:
© 2016 Watson, Carlson, Largaespada, Hackett and Fahrenkrug.
PY - 2016/5/9
Y1 - 2016/5/9
N2 - Over the past decade, the technology to engineer genetically modified swine has seen many advancements, and because their physiology is remarkably similar to that of humans, swine models of cancer may be extremely valuable for preclinical safety studies as well as toxicity testing of pharmaceuticals prior to the start of human clinical trials. Hence, the benefits of using swine as a large animal model in cancer research and the potential applications and future opportunities of utilizing pigs in cancer modeling are immense. In this review, we discuss how pigs have been and can be used as a biomedical models for cancer research, with an emphasis on current technologies. We have focused on applications of precision genetics that can provide models that mimic human cancer predisposition syndromes. In particular, we describe the advantages of targeted gene-editing using custom endonucleases, specifically TALENs and CRISPRs, and transposon systems, to make novel pig models of cancer with broad preclinical applications.
AB - Over the past decade, the technology to engineer genetically modified swine has seen many advancements, and because their physiology is remarkably similar to that of humans, swine models of cancer may be extremely valuable for preclinical safety studies as well as toxicity testing of pharmaceuticals prior to the start of human clinical trials. Hence, the benefits of using swine as a large animal model in cancer research and the potential applications and future opportunities of utilizing pigs in cancer modeling are immense. In this review, we discuss how pigs have been and can be used as a biomedical models for cancer research, with an emphasis on current technologies. We have focused on applications of precision genetics that can provide models that mimic human cancer predisposition syndromes. In particular, we describe the advantages of targeted gene-editing using custom endonucleases, specifically TALENs and CRISPRs, and transposon systems, to make novel pig models of cancer with broad preclinical applications.
KW - Cancer genetics
KW - Genetically engineered swine
KW - Genome engineering
KW - Preclinical cancer models
KW - Swine models
UR - http://www.scopus.com/inward/record.url?scp=84975263430&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84975263430&partnerID=8YFLogxK
U2 - 10.3389/fgene.2016.00078
DO - 10.3389/fgene.2016.00078
M3 - Review article
C2 - 27242889
AN - SCOPUS:84975263430
VL - 7
JO - Frontiers in Genetics
JF - Frontiers in Genetics
SN - 1664-8021
IS - MAY
M1 - 78
ER -
