Engineered streptomyces platensis strains that overproduce antibiotics platensimycin and platencin

Michael J. Smanski, Ryan M. Peterson, Scott R. Rajski, Ben Shen

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

Platensimycin, which is isolated from Streptomyces platensis MA7327, and platencin, which is isolated from S. platensis MA7339, are two recently discovered natural products that serve as important antibiotic leads. Here we report on the identification of S. platensis MA7327 as a dual producer of both platensimycin and platencin. A PCR-based approach was used to locate and clone the locus involved in platensimycin and platencin production, including ptmRl, which encodes a putative GntR-like transcriptional regulator. Deletion of this gene from the producing organism allowed us to isolate strains that overproduce platensimycin and platencin with yields of 323 ± 29 mg/liter and 255 ± 30 mg/liter, respectively. These results illustrate the effectiveness of genetic manipulation for the rational engineering of improvements in titers.

Original languageEnglish (US)
Pages (from-to)1299-1304
Number of pages6
JournalAntimicrobial agents and chemotherapy
Volume53
Issue number4
DOIs
StatePublished - Apr 2009

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