Engineered scaffold-free tendon tissue produced by tendon-derived stem cells

Ming Ni; Yun Feng Rui; Qi Tan; Yang Liu; Liang Liang Xu; Kai Ming Chan; Yan Wang; Gang Li

doi:10.1016/j.biomaterials.2012.11.046

Engineered scaffold-free tendon tissue produced by tendon-derived stem cells

Ming Ni, Yun Feng Rui, Qi Tan, Yang Liu, Liang Liang Xu, Kai Ming Chan, Yan Wang, Gang Li

Research output: Contribution to journal › Article › peer-review

128 Scopus citations

Abstract

Most of the exogenous biomaterials for tendon repair have limitations including lower capacity for inducing cell proliferation and differentiation, poorer biocompatibility and remodeling potentials. To avoid these shortcomings, we intend to construct an engineered tendon by stem cells and growth factors without exogenous scaffolds. In this study, we produced an engineered scaffold-free tendon tissue (ESFTT) in vitro and investigated its potentials for neo-tendon formation and promoting tendon healing in vivo. The ESFTT, produced via tendon-derived stem cells (TDSCs) by treatment of connective tissue growth factor (CTGF) and ascorbic acid in vitro, was characterized by histology, qRT-PCR and immunohistochemistry methods. After ESFTT implanted into the nude mouse, the in vivo fluorescence imaging, histology and immunohistochemistry examinations showed neo-tendon formation. In a rat patellar tendon window injury model, the histology, immunohistochemistry and biomechanical testing data indicated ESFTT could significantly promote tendon healing. In conclusion, this is a proof-of-concept study demonstrating that ESFTT could be a potentially new approach for tendon repair and regeneration.

Original language	English (US)
Pages (from-to)	2024-2037
Number of pages	14
Journal	Biomaterials
Volume	34
Issue number	8
DOIs	https://doi.org/10.1016/j.biomaterials.2012.11.046
State	Published - Mar 2013
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank Dr. Pauline Po-Yee Lui for her useful discussion and supports for this project. This work was supported by equipment/resources donated by the Hong Kong Jockey Club Charities Trust to Kai-Ming Chan. These works were also supported in part by the National Basic Science and Development Program, PR China (973 Program, 2012CB518105 ); and Hong Kong Government Research Grant Council, General Research Fund (Grant No: CUHK460710 ) to Kai-Ming Chan.

Keywords

Cell sheet
Connective tissue growth factor
Scaffold-free
Tendon tissue engineering
Tendon-derived stem cells
Tenogenic differentiation

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10.1016/j.biomaterials.2012.11.046

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title = "Engineered scaffold-free tendon tissue produced by tendon-derived stem cells",

abstract = "Most of the exogenous biomaterials for tendon repair have limitations including lower capacity for inducing cell proliferation and differentiation, poorer biocompatibility and remodeling potentials. To avoid these shortcomings, we intend to construct an engineered tendon by stem cells and growth factors without exogenous scaffolds. In this study, we produced an engineered scaffold-free tendon tissue (ESFTT) in vitro and investigated its potentials for neo-tendon formation and promoting tendon healing in vivo. The ESFTT, produced via tendon-derived stem cells (TDSCs) by treatment of connective tissue growth factor (CTGF) and ascorbic acid in vitro, was characterized by histology, qRT-PCR and immunohistochemistry methods. After ESFTT implanted into the nude mouse, the in vivo fluorescence imaging, histology and immunohistochemistry examinations showed neo-tendon formation. In a rat patellar tendon window injury model, the histology, immunohistochemistry and biomechanical testing data indicated ESFTT could significantly promote tendon healing. In conclusion, this is a proof-of-concept study demonstrating that ESFTT could be a potentially new approach for tendon repair and regeneration.",

keywords = "Cell sheet, Connective tissue growth factor, Scaffold-free, Tendon tissue engineering, Tendon-derived stem cells, Tenogenic differentiation",

author = "Ming Ni and Rui, {Yun Feng} and Qi Tan and Yang Liu and Xu, {Liang Liang} and Chan, {Kai Ming} and Yan Wang and Gang Li",

note = "Funding Information: The authors would like to thank Dr. Pauline Po-Yee Lui for her useful discussion and supports for this project. This work was supported by equipment/resources donated by the Hong Kong Jockey Club Charities Trust to Kai-Ming Chan. These works were also supported in part by the National Basic Science and Development Program, PR China (973 Program, 2012CB518105 ); and Hong Kong Government Research Grant Council, General Research Fund (Grant No: CUHK460710 ) to Kai-Ming Chan.",

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AU - Chan, Kai Ming

AU - Wang, Yan

AU - Li, Gang

N1 - Funding Information: The authors would like to thank Dr. Pauline Po-Yee Lui for her useful discussion and supports for this project. This work was supported by equipment/resources donated by the Hong Kong Jockey Club Charities Trust to Kai-Ming Chan. These works were also supported in part by the National Basic Science and Development Program, PR China (973 Program, 2012CB518105 ); and Hong Kong Government Research Grant Council, General Research Fund (Grant No: CUHK460710 ) to Kai-Ming Chan.

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N2 - Most of the exogenous biomaterials for tendon repair have limitations including lower capacity for inducing cell proliferation and differentiation, poorer biocompatibility and remodeling potentials. To avoid these shortcomings, we intend to construct an engineered tendon by stem cells and growth factors without exogenous scaffolds. In this study, we produced an engineered scaffold-free tendon tissue (ESFTT) in vitro and investigated its potentials for neo-tendon formation and promoting tendon healing in vivo. The ESFTT, produced via tendon-derived stem cells (TDSCs) by treatment of connective tissue growth factor (CTGF) and ascorbic acid in vitro, was characterized by histology, qRT-PCR and immunohistochemistry methods. After ESFTT implanted into the nude mouse, the in vivo fluorescence imaging, histology and immunohistochemistry examinations showed neo-tendon formation. In a rat patellar tendon window injury model, the histology, immunohistochemistry and biomechanical testing data indicated ESFTT could significantly promote tendon healing. In conclusion, this is a proof-of-concept study demonstrating that ESFTT could be a potentially new approach for tendon repair and regeneration.

AB - Most of the exogenous biomaterials for tendon repair have limitations including lower capacity for inducing cell proliferation and differentiation, poorer biocompatibility and remodeling potentials. To avoid these shortcomings, we intend to construct an engineered tendon by stem cells and growth factors without exogenous scaffolds. In this study, we produced an engineered scaffold-free tendon tissue (ESFTT) in vitro and investigated its potentials for neo-tendon formation and promoting tendon healing in vivo. The ESFTT, produced via tendon-derived stem cells (TDSCs) by treatment of connective tissue growth factor (CTGF) and ascorbic acid in vitro, was characterized by histology, qRT-PCR and immunohistochemistry methods. After ESFTT implanted into the nude mouse, the in vivo fluorescence imaging, histology and immunohistochemistry examinations showed neo-tendon formation. In a rat patellar tendon window injury model, the histology, immunohistochemistry and biomechanical testing data indicated ESFTT could significantly promote tendon healing. In conclusion, this is a proof-of-concept study demonstrating that ESFTT could be a potentially new approach for tendon repair and regeneration.

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KW - Tendon tissue engineering

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KW - Tenogenic differentiation

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Engineered scaffold-free tendon tissue produced by tendon-derived stem cells

Abstract

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Keywords

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