Endotoxin lipopolysaccharide from Escherichia coli and its effects on the phagocytic function of systemic and pulmonary macrophages in turkeys.

D. A. Emery; K. V. Nagaraja; V. Sivanandan; B. W. Lee; C. L. Zhang; J. A. Newman

doi:10.2307/1591627

Endotoxin lipopolysaccharide from Escherichia coli and its effects on the phagocytic function of systemic and pulmonary macrophages in turkeys.

D. A. Emery, K. V. Nagaraja, V. Sivanandan, B. W. Lee, C. L. Zhang, J. A. Newman

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The effect of Escherichia coli lipopolysaccharide (LPS) on the competence of pulmonary macrophages and phagocytic cells from the systemic circulation of turkeys was examined using luminol-enhanced zymosan-stimulated chemiluminescence. The results showed a rapid and accelerated oxidative burst in both systemic and pulmonary macrophages in LPS-treated turkeys that was significantly greater than in untreated controls. However, this increased oxidative metabolism induced by LPS was not associated with enhanced intracellular bacterial killing by pulmonary macrophages. Turkeys treated with LPS showed a highly significant decrease in pulmonary bactericidal activity against Staphylococcus aureus challenge, indicating a defect in pulmonary macrophage function induced by LPS.

Original language	English (US)
Pages (from-to)	901-909
Number of pages	9
Journal	Avian diseases
Volume	35
Issue number	4
DOIs	https://doi.org/10.2307/1591627
State	Published - Jan 1 1991

Publisher link

10.2307/1591627

Find It

Find It at U of M Libraries

Cite this

@article{8a74afe1eced4a8d8d05e364bd543348,

title = "Endotoxin lipopolysaccharide from Escherichia coli and its effects on the phagocytic function of systemic and pulmonary macrophages in turkeys.",

abstract = "The effect of Escherichia coli lipopolysaccharide (LPS) on the competence of pulmonary macrophages and phagocytic cells from the systemic circulation of turkeys was examined using luminol-enhanced zymosan-stimulated chemiluminescence. The results showed a rapid and accelerated oxidative burst in both systemic and pulmonary macrophages in LPS-treated turkeys that was significantly greater than in untreated controls. However, this increased oxidative metabolism induced by LPS was not associated with enhanced intracellular bacterial killing by pulmonary macrophages. Turkeys treated with LPS showed a highly significant decrease in pulmonary bactericidal activity against Staphylococcus aureus challenge, indicating a defect in pulmonary macrophage function induced by LPS.",

author = "Emery, {D. A.} and Nagaraja, {K. V.} and V. Sivanandan and Lee, {B. W.} and Zhang, {C. L.} and Newman, {J. A.}",

year = "1991",

month = jan,

day = "1",

doi = "10.2307/1591627",

language = "English (US)",

volume = "35",

pages = "901--909",

journal = "Avian Diseases",

issn = "0005-2086",

publisher = "American Association of Avian Pathologists",

number = "4",

}

TY - JOUR

T1 - Endotoxin lipopolysaccharide from Escherichia coli and its effects on the phagocytic function of systemic and pulmonary macrophages in turkeys.

AU - Emery, D. A.

AU - Nagaraja, K. V.

AU - Sivanandan, V.

AU - Lee, B. W.

AU - Zhang, C. L.

AU - Newman, J. A.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - The effect of Escherichia coli lipopolysaccharide (LPS) on the competence of pulmonary macrophages and phagocytic cells from the systemic circulation of turkeys was examined using luminol-enhanced zymosan-stimulated chemiluminescence. The results showed a rapid and accelerated oxidative burst in both systemic and pulmonary macrophages in LPS-treated turkeys that was significantly greater than in untreated controls. However, this increased oxidative metabolism induced by LPS was not associated with enhanced intracellular bacterial killing by pulmonary macrophages. Turkeys treated with LPS showed a highly significant decrease in pulmonary bactericidal activity against Staphylococcus aureus challenge, indicating a defect in pulmonary macrophage function induced by LPS.

AB - The effect of Escherichia coli lipopolysaccharide (LPS) on the competence of pulmonary macrophages and phagocytic cells from the systemic circulation of turkeys was examined using luminol-enhanced zymosan-stimulated chemiluminescence. The results showed a rapid and accelerated oxidative burst in both systemic and pulmonary macrophages in LPS-treated turkeys that was significantly greater than in untreated controls. However, this increased oxidative metabolism induced by LPS was not associated with enhanced intracellular bacterial killing by pulmonary macrophages. Turkeys treated with LPS showed a highly significant decrease in pulmonary bactericidal activity against Staphylococcus aureus challenge, indicating a defect in pulmonary macrophage function induced by LPS.

UR - http://www.scopus.com/inward/record.url?scp=0026236462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026236462&partnerID=8YFLogxK

U2 - 10.2307/1591627

DO - 10.2307/1591627

M3 - Article

C2 - 1838477

AN - SCOPUS:0026236462

SN - 0005-2086

VL - 35

SP - 901

EP - 909

JO - Avian Diseases

JF - Avian Diseases

IS - 4

ER -

Endotoxin lipopolysaccharide from Escherichia coli and its effects on the phagocytic function of systemic and pulmonary macrophages in turkeys.

Abstract

Publisher link

Find It

Other files and links

Fingerprint

Cite this