TY - JOUR
T1 - Endothelial Cell Heterogeneity
T2 - Antioxidant Profiles Determine Vulnerability to Oxidant Injury
AU - Vercellotti, Gregory M.
AU - Dobson, Martha
AU - Schorer, Anna E.
AU - Moldow, Charles F.
N1 - Funding Information:
This work was supported by NIH Grants AM01387 and ML33793 and the Veterans Administration Research Service.
PY - 1988/2
Y1 - 1988/2
N2 - Human umbilical vein endothelial cells were more sensitive to hydrogen peroxide lysis than cow pulmonary artery endothelial cells. Conversely, activated neutrophils which utilize hydrogen peroxide-mediated cell cytotoxicity cell mechanisms were more toxic to the cow pulmonary artery cells. This discordance was not related to neutrophil adhesion to either cell type or cell passage number. The antioxidant profiles of the endothelial cells revealed that cow pulmonary artery cells were rich in catalase to consume bolus hydrogen peroxide presented to them, while human umbilical vein endothelial cells utilize glutathione peroxidase-linked mechanisms to detoxify a slower more sustained release of hydrogen peroxide generated by neutrophils. Endothelial cells from different species and sites may utilize diversified antioxidant protective mechanisms.
AB - Human umbilical vein endothelial cells were more sensitive to hydrogen peroxide lysis than cow pulmonary artery endothelial cells. Conversely, activated neutrophils which utilize hydrogen peroxide-mediated cell cytotoxicity cell mechanisms were more toxic to the cow pulmonary artery cells. This discordance was not related to neutrophil adhesion to either cell type or cell passage number. The antioxidant profiles of the endothelial cells revealed that cow pulmonary artery cells were rich in catalase to consume bolus hydrogen peroxide presented to them, while human umbilical vein endothelial cells utilize glutathione peroxidase-linked mechanisms to detoxify a slower more sustained release of hydrogen peroxide generated by neutrophils. Endothelial cells from different species and sites may utilize diversified antioxidant protective mechanisms.
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U2 - 10.3181/00379727-187-42652
DO - 10.3181/00379727-187-42652
M3 - Article
C2 - 3340627
AN - SCOPUS:0023832492
SN - 0037-9727
VL - 187
SP - 181
EP - 189
JO - Proceedings of the Society for Experimental Biology and Medicine
JF - Proceedings of the Society for Experimental Biology and Medicine
IS - 2
ER -