Endoplasmic Reticulum Stress and Cellular Homeostasis in Genetically Engineered Porcine Donors for Xenotransplantation

Nora Hosny, Joseph Sushil Rao, Christopher Burlak

Research output: Contribution to journalReview articlepeer-review

Abstract

Genetically engineered pigs with multiple gene deletions and insertions are predicted to extend porcine to human xenograft survival. Several genes have been successfully knocked out and inserted, yet more have failed to produce viable animals for unexplained reasons. The effects of gene editing on cellular homeostasis may be the cause of reduced embryo fitness, failed pregnancies, or poor piglet viability. The elements of cellular dysfunction such as endoplasmic reticulum stress and oxidative stress induced by gene editing may additively affect the quality of genetically engineered cells to be used for cloning. Evaluating the impact of each gene edit on cellular fitness for cloning will allow researchers to maintain the cellular homeostasis of engineered cells that were validated as candidates for cloning and the production of porcine organ donors.

Original languageEnglish (US)
Pages (from-to)387-396
Number of pages10
JournalExperimental and Clinical Transplantation
Volume21
Issue number5
DOIs
StatePublished - May 2023

Bibliographical note

Publisher Copyright:
© Başkent University 2023 Printed in Turkey. All Rights Reserved.

Keywords

  • Endoplasmic reticulum stress
  • Genetic engineering
  • Nonsense-mediated RNA decay
  • Organ Donation
  • Transplantation

PubMed: MeSH publication types

  • Journal Article
  • Review

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