TY - JOUR
T1 - Endometrial stromal cells in culture
T2 - An attempt to understand the genesis and biologic activity of uterine sarcomas
AU - Walton, Leslie A.
AU - Siegfried, Jill M.
AU - Nelson, Karen G.
AU - Siegal, Gene
AU - Kaufman, David G.
N1 - Funding Information:
’ This work was supported by Contract NOI-CP75 956 and Grant ROI-CA31733 from the National Cancer Institute. ’ To whom reprint requests should be addressed.
PY - 1986/6
Y1 - 1986/6
N2 - The genesis of uterine sarcomas in vivo has been difficult to elucidate. The reasons are multifactorial and are compounded by the rarity of these tumors. In an effort to understand the biologic activity of this disease, normal endometrial tissue was subjected to tissue culture, histochemical study, and hormonal manipulation. Protein markers were used to differentiate endometrial epithelium from endometrial stromal cells in culture. The endometrial stromal cells grew rapidly following subculturing and were responsive to hormonal manipulation. When the stromal cells in culture were treated with the carcinogen N-methylN′-nitro-N-nitrosoguanidine (MNNG), the resultant morphologic changes mimicked uterine sarcomas grown in culture. These changes appeared to occur in a sequential manner, somewhat analogous to changes occurring in the endometrial epithelium as it undergoes transformation to endometrial adenocarcinoma. These studies, therefore, may serve as a model system in understanding the genesis and biologic activity of uterine sarcomas.
AB - The genesis of uterine sarcomas in vivo has been difficult to elucidate. The reasons are multifactorial and are compounded by the rarity of these tumors. In an effort to understand the biologic activity of this disease, normal endometrial tissue was subjected to tissue culture, histochemical study, and hormonal manipulation. Protein markers were used to differentiate endometrial epithelium from endometrial stromal cells in culture. The endometrial stromal cells grew rapidly following subculturing and were responsive to hormonal manipulation. When the stromal cells in culture were treated with the carcinogen N-methylN′-nitro-N-nitrosoguanidine (MNNG), the resultant morphologic changes mimicked uterine sarcomas grown in culture. These changes appeared to occur in a sequential manner, somewhat analogous to changes occurring in the endometrial epithelium as it undergoes transformation to endometrial adenocarcinoma. These studies, therefore, may serve as a model system in understanding the genesis and biologic activity of uterine sarcomas.
UR - https://www.scopus.com/pages/publications/0022515093
UR - https://www.scopus.com/pages/publications/0022515093#tab=citedBy
U2 - 10.1016/0090-8258(86)90033-8
DO - 10.1016/0090-8258(86)90033-8
M3 - Article
C2 - 3710269
AN - SCOPUS:0022515093
SN - 0090-8258
VL - 24
SP - 247
EP - 257
JO - Gynecologic oncology
JF - Gynecologic oncology
IS - 2
ER -
